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To determine which color of light will provide the best possible resolution for studying cell structure under a light microscope, we need to consider the relationship between wavelength and resolution.1. Understand Resolution:
Images were captured by using a digital camera (DP73, Olympus) under a light microscope (BX53, Olympus). The pathological lesions and SARS-CoV-2 spike protein were quantified by two pathologists independently. Briefly, three phases of DAD were evaluated according to the histopathological lesion ...
colcemid (Invitrogen, New York, USA) treated, hypotonic N/TERT keratinocytes were fixed by methanol:acetic acid (3:1) before Giemsa-staining on glass slides. Metaphases were analysed under light microscope by Clinical Assistant (RVC, Baarn, The Netherlands). Additional short tandem repeat (STR) ...
Build live-cell experiments for your imaging platform with an incubator or microscope Play Mute Current Time 0:00 / Duration 0:00 Loaded: 0% SharePicture-in-PictureFullscreen This is a modal window. The Playback API request fail...
cells were fixed with 4% paraformaldehyde and permeabilized with 0.3%Triton X-100. The cells was dyed using click reaction solution and captured under a fluorescence microscope. DAPI was used to stain the cell nuclei. Three random fields of each section were captured with EVOS FL Auto Cell Imag...
If your samples are in plastic vessels and you image samples using transmitted light, use either achromat or fluorite LWD objectives. If your samples are under glass coverslips and you want the best image quality, use apochromatic CC or fluorite CC objectives.EVOS...
(ApogeeKX4, Apogee Instruments) and light source maintain the same position; the motor-controlled xy stage of the inverted fluorescence microscope (Leica DMIRE2, Leica Microsystems; x20 air lens; numerical aperture, 0.7) moves in between fields of view. Images with a resolution of 2018 ×...
An operator monitors the ‘floating’ or circularity of the cell population at regular times with the microscope10, to decide when to stop the enzymatic reaction. Cells undergo specific morphological changes upon detachment, such as an increased circularity/roundness and intensity, which can form the...
performed [32]. At the end of the for 28 days of cell growth, the cell culture medium was replaced with fresh medium containing 0.5 mg/mL of (MTT) and the cells were incubated for 4 h. Then, culture medium was aspirated, and MTT-formazan-stained cells were imaged under light ...