The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.Features of the Cell Surface Protein Biotinylation and Isolation Kit include:• Improved performance—kit and protocol have been ...
The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins. This easy-to-use kit provides all of the necessary components and procedure for optimal labeling and subsequent isolation of cel...
Protocol summary for the Cell Surface Protein Biotinylation and Isolation Kit Western blot analysis of enriched cell surface proteins Samples were prepared in duplicate from two cell lines using the Cell Surface Protein Biotinylation and Isolation Kit. Flow-through (F), elution (E), and bead boil ...
规格:1 kit 价格:5170 产品介绍 技术参数 相关产品 产品介绍 The FluoReporter® Cell-Surface Biotinylation Kit provides a convenient method to label proteins exposed on the cell surface including, but not limited to, membrane proteins. The kit supplies 5 vials of biotin-XX SSE, and each vial ca...
Flexibility in Cell Isolation Dynabeads Streptavidin - Products and Applications Product Information CELLection Biotin Binder Kit 常見問答集 (常見問題) The indirect technique is chosen when the antigen targeted by the primary antibody is expressed in low density on the target cell surface. This is due...
单元格可以调节其通过内吞作用后的细胞表面蛋白有效地隐藏在细胞质细胞膜上的特定蛋白量。一次在一个单元格中,这种表面蛋白可以摧毁或者”回收”回膜。细胞表面生物素化检测提供了一种方法来研究这些现象的研究人员。该技术利用的小分子生物素,可以添加标签表面蛋白,然后化学分解的衍生物。然而,如果表面蛋白 endocytosed...
This chapter focuses on cell surface biotinylation and other techniques for determination of surface polarity of epithelial monolayers. The proteins present on the surface of the filter-grown monolayers can be selectively modified by the water soluble cell-impermeable biotin analog sulfo-NHSbiotin. ...
Biotinylation and giant plasma membrane vesicle assays Biotinylation assays were performed on overnight serum starved cells using the Cell Surface Protein Isolation Kit (Pierce) according to the manufacturer’s instructions. For biotinylation assays to measure insulin induced INSR membrane trafficking, HeLa...
Total RNA was isolated from 1 × 106 cells by using a High Pure RNA Isolation Kit (Roche). The cDNA was synthesized from 1 μg of the total RNA with an oligo (dT)20 primer and SuperScript II reverse transcriptase (Invitrogen), according to the manufacturer's instructions. Subsequently, ...
Initial contact between the opportunistic fungal pathogen Candida albicans and host tissue occurs at the cell surface. Biotin derivatives have been used to label the cell-surface proteins of yeasts, with labelled proteins subsequently detected by avidin–reporter conjugates. Previous work has indicated th...