1991. Biotinylation of cell surface proteins in carrot suspen- sion cells. J. Plant Physiol. 139:45-51.Grimes, H.D. (1991) Biotinylation of cell surface proteins in carrot suspension cells. J. Plant Physiol. 139, 45.Grimes HD (1991) Biotinylation of cell surface proteins in carrot ...
Biotinylation of Cell Surface Proteins in Carrot Suspension Cells The ability of sulfosuccinimidobiotin to label proteins associated with the plasma membrane and cell wall of intact carrot suspension cells was addressed. ... Grimes,D Howard - 《Journal of Plant Physiology》 被引量: 13发表: 1991...
Cell surface proteins play a very important role in physiology and pathology and are receiving increased attention by the pharmaceutical industry as valuable targets for development of new therapeutics. However, owing to the very nature of this category of proteins, their comprehensive study remains an...
Cell-surface Biotinylation Assay 1. Overview Labeling of cell surface proteins with biotin presents a powerful tool to study cellular transport pathways involved in regulating membrane proteins. A cell maintains a tightly regulated set of proteins at the surface, so that it can receive and respond ...
Our 48-page Avidin-Biotin Technical Handbook brings together everything needed to biotinylate, purify or detect proteins. Featured products include cell-surface protein biotinylation and purification kits, antibody labeling and new photo-reactive biotinylation reagents....
Cell surface proteins represent a key subset of cellular proteins and play major roles in signal transduction, cell adhesion, and ion transport. These proteins are challenging to efficiently extract and isolate due to their multiple membrane-spanning domains. Often, the harsh detergents and conditions...
Cell surface proteinsand their subsequent trafficking can be assessed in cell populations via a variety of protocols that involve the incorporation of abiotinlabel. Thebiotinylationprotocols described here are based upon the use of EZ-Link® Sulfo-NHS-SS-Biotin, which is a thiol-cleavable, amine...
The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins. This easy-to-use kit provides all of the necessary components and procedure for optimal labeling and subsequent isolation of cel...
Specific labeling of cell surface proteins is another useful application for this water-soluble and membrane-impermeable reagent. The No-Weigh NHS-PEO4-Biotin is packaged in convenient two-milligram 8、 aliquots, eliminating difficulties associated with weighing small quantities of reagent. Pierce ...
Our 48-page Avidin-Biotin Technical Handbook brings together everything needed to biotinylate, purify or detect proteins. Featured products include cell-surface protein biotinylation and purification kits, antibody labeling and new photo-reactive biotinylation reagents...