单元格可以调节其通过内吞作用后的细胞表面蛋白有效地隐藏在细胞质细胞膜上的特定蛋白量。一次在一个单元格中,这种表面蛋白可以摧毁或者”回收”回膜。细胞表面生物素化检测提供了一种方法来研究这些现象的研究人员。该技术利用的小分子生物素,可以添加标签表面蛋白,然后化学分解的衍生物。然而,如果表面蛋白 endocytosed...
品牌:Thermo Fisher 货号:F20650 规格:1 kit 价格:5170 产品介绍 技术参数 相关产品 产品介绍 The FluoReporter® Cell-Surface Biotinylation Kit provides a convenient method to label proteins exposed on the cell surface including, but not limited to, membrane proteins. The kit supplies 5 vials of bi...
Cell-surface Biotinylation Assay Cell-surface Biotinylation Assay 1. Overview Labeling of cell surface proteins with biotin presents a powerful tool to study cellular transport pathways involved in regulating membrane proteins. A cell maintains a tightly regulated set of proteins at the surface, so that...
Tanaka K, Yokoi S, Morimoto K, Iwata T, Nakamoto Y, Nakayama K, Koyama K, Fujiwara T, Fukase K (2012) Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling. Bioorg Med Chem 20(6):1865-1868...
Pierce Cell Surface Biotinylation and Isolation Kit / 细胞膜蛋白生物素化和分离试剂盒产品类型:蛋白提取/纯化品牌:Thermo Fisher货号:A44390 规格:8 samples 价格:5681加入购物车 产品介绍 技术参数 相关产品 产品介绍 The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the...
Cell surface proteins from A549 cells were isolated using Cell Surface Protein Biotinylation and Isolation Kit (Cat. No. A44390) or Pierce Cell Surface Protein Isolation Kit (Cat. No. 89881) in duplicate. A549 whole cell lysate was analyzed as a no-enrichment control. Samples were then prepare...
About this protocol Cite this protocol Elia, G. (2012). Cell Surface Protein Biotinylation for SDS-PAGE Analysis. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_29...
Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor ...
Biotinylation of MV4-11 cell surface proteins followed by immunoblotting of the biotinylated proteins showed that full-length (106 kDa) and truncated forms... S Soundararajan,L Wang,V Sridharan,... - 《Molecular Pharmacology》 被引量: 181发表: 2009年 Metabolic biotinylation of cell surface re...
To label surface expression of endogenous TG2 and exogenous GST-C277A/TG2, HUVEC cells were treated with TNFα for 5 hours and 50 nM of GST-C277A/TG2 cells was added at the final hour. Cell surface proteins were labeled using FluoReporter™ Cell-Surface Biotinylation Kit (ThermoFi...