Cell-surface Biotinylation Assay Cell-surface Biotinylation Assay 1. Overview Labeling of cell surface proteins with biotin presents a powerful tool to study cellular transport pathways involved in regulating membrane proteins. A cell maintains a tightly regulated set of proteins at the surface, so that...
单元格可以调节其通过内吞作用后的细胞表面蛋白有效地隐藏在细胞质细胞膜上的特定蛋白量。一次在一个单元格中,这种表面蛋白可以摧毁或者”回收”回膜。细胞表面生物素化检测提供了一种方法来研究这些现象的研究人员。该技术利用的小分子生物素,可以添加标签表面蛋白,然后化学分解的衍生物。然而,如果表面蛋白 endocytosed...
A Cell Surface Biotinylation Assay to Reveal Membrane-associated Neuronal Cues: Negr1 Regulates Dendritic Arborizationdoi:10.1074/mcp.M113.031716Francesca PischeddaJoanna SzczurkowskaMaria CirnaruGiovanni Piccoli
Cite this protocol Elia, G. (2012). Cell Surface Protein Biotinylation for SDS-PAGE Analysis. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_29 Download citation .R...
Protocol summary for the Cell Surface Protein Biotinylation and Isolation Kit Western blot analysis of enriched cell surface proteins Samples were prepared in duplicate from two cell lines using the Cell Surface Protein Biotinylation and Isolation Kit. Flow-through (F), elution (E), and bead boil ...
The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.Features of the Cell Surface Protein Biotinylation and Isolation Kit include:• Improved performance—kit and protocol have been ...
Flow cytometry, immunofluorescent staining, and cell surface biotinylation established that full-length, 14-kDa Ecrg4 was localized on PMN and monocyte cell... A Baird,R Coimbra,X Dang,... - 《Journal of Leukocyte Biology》 被引量: 53发表: 2012年 A protocol to effectively create single cel...
Protocol summary for the Cell Surface Protein Biotinylation and Isolation Kit Western blot analysis of enriched cell surface proteinsSamples were prepared in duplicate from two cell lines using the Cell Surface Protein Biotinylation and Isolation Kit. Flow-through (F), elution (E), and bead boil (...
Cell Invasion Assays—The Biocoat Matrigel invasion chamber system (BD Biosciences) was used to assay the invasive potential of cells cultured in the presence or absence of IFNγ, employing the protocol of Falcone et al. (25). To evaluate the effect of antibody blockage, the Matrigel was re...
Surface biotinylation assay Cancer cells were washed three times in ice-cold PBS and surface-labelled at 4°C with 0.3 mg/mL NHS-LC-biotin (Thermo Scientific) in PBS for 30 min. Labeled cells were washed four times in ice-cold PBS, and excess biotin was removed by incubation with RPMI ...