To extract the total protein, THP-1 cells were lysed in RIPA buffer (Cell Signaling Technology, Inc., Danvers, MA, USA) containing 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na2-EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 m...
An antibody against Beclin-1 (cat. no. 3495T; 1:1000) was obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). β-Actin (cat. no. bs-0061R; 1:5000) was purchased from Bioss Biotechnology (Beijing, China), and goat anti-mouse IgG (cat. no. bs-0295GS; 1:2000) ...
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(Cell Signaling Technology, Inc., Danvers, MA; #CS 9271s) with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Cell Signaling Technology, Inc., Danvers, MA; #A11070); FITC-conjugated monoclonal antibody against Bcl-2 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA; #sc-7382); and ...
Diabetic nephropathy (DN) is one of the serious chronic microvascular complications of diabetes, and leads to the increased morbidity and mortality in diabetic patients. Gasdermin E (GSDME)-dependent pyroptosis signaling pathway plays important roles in
(Cell Signaling Technology, Danvers, MA, http://www.cellsignal.com/), GF109203X (GFX; Tocris Bioscience), U0126 (Promega, Madison, WI, www.promega.com), (2′Z,3′E)−6-Bromoindirubin-3′-oxime (BIO; Wako Pure Chemical Industries), epidermal growth factor (EGF; R&D Systems), ...
sc-1303), rabbit anti-Sdf1 (1/50, Santa Cruz Biotechnology, sc-28876), rabbit anti-Integrinβ1 (1/500, Chemicon, Billerica, MA, USA, AB1952), rabbit anti-E-cadherin (1/200, Abcam, ab15148), and rabbit anti-Vimentin (1/200, Cell Signaling Technology, Danvers, MA, USA, #3932)...
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Immunohistochemistry was performed using standard protocols with the following antibodies: C/EBPalpha (1:100) and active beta-catenin (1:200, both from Cell Signaling Technology, Inc., Danvers, MA, USA). Cell culture Bone marrow cells from tibia and femur of 10–12-week-old mice were ...
AKT-2, CDK6, c-Abl, Src and β-actin antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). MiRNA and mRNA profiling and miRNA target prediction It was previously established that overexpression of ING1b inhibits cell proliferation (Garkavtsev et al, 1996) and ...