Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 美国，马萨诸塞州，波士顿市，丹佛斯镇
The following primary antibodies were used at indicated dilution: rabbit monoclonal to phospho-RIPK1 (Ser166, clone D1L3S) diluted 1:1 000 (65746, Cell Signaling Technology, Danvers, MA, USA); rabbit polyclonal to PARP1 diluted 1:1 000 (ab137653, Abcam); mouse monoclonal to SQSTM1/p62...
美国波士顿 Cell Signaling Technology (CST) 公司的核心技术是生产”聪明的抗体”。主要是针对活化状态蛋白，包括磷酸化，乙酰化，特异性剪切，亚硝基化以及其他形式活化的蛋白。 这一类抗体是用于检测在细胞调解中起重要作用的活化的蛋白质。同时致力于开发新型的研究信号传导的方法，提供有关信号传导研究中...
(Tyr705, 9145, 1:2000 for WB, 1:100 for IF) were from Cell Signaling (Danvers, MA, USA); Ki67 (RB-9043-P1, 1:500 for IF) was from Thermo (Fremont, CA, USA); Glyceraldehyde-3-phosphate dehydrogenase (ABS16, 1:2500 for WB), GFAP (AB5804, 1:2000 for IF) and Olig2 (AB...
The sections were incubated with anti-GM-CSF antibodies (1:100, MP1-22E9, catalog 2131415, Invitrogen) overnight at 4 °C and further incubated with the corresponding fluorochrome-conjugated secondary antibody (1:1,000, Cell Signaling Technology, Danvers, MA, USA) for 50 min. After counter...
All antibodies were purchased commercially: anti-β-actin (I-19-R, Santa Cruz, CA), anti-DNA-PKcs (H-163, Santa Cruz, CA), γH2AX (05-636, Upstate Biotechnology, Charlottesville, VA), anti-GSK3β (#9332, Cell Signaling, Danvers, MA), anti-phospho-GSK3β (Ser-9, #9336, Cell ...
Primary antibodies used were anti-CleavedCaspase-3 (Asp175) (Cell Signaling, Danvers, MA) and anti pHH3 (Ser10) (Millipore, Billerica, MA). Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA) was used as secondary antibody. A tyramide signal amplification kit (...
Antibodies directed against caspase-3, caspase-9, apoptosis protease-activating factor-1 (Apaf-1), cleaved caspase-3, cleaved caspase-9 were purchased from Cell Signaling Technology, Danvers, MA. Anti LC3A/B, Fis1, Drp1, p-Drp1, and β-actin were purchased from Abcam, Cambridge, MA. Cell...
to 1:250 (Cell Signaling, Danvers, MA, USA), anti-mouse p63 diluted to 1:100 (Santa Cruz Biotechnology, Dallas, TX, USA), and anti-mouse MUC1 and MUC4 diluted to 1:200 (both Abcam, Cambridge, CA, USA), anti-mouse NKX2-1 diluted to 1:100 (Lab Vision Corporation, Fremont, CA,...
(Cell Signaling Technology, #5684), EAAT2 antibody (Cell Signaling Technology, #3838), goat anti-rabbit, and goat anti-mouse secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA95060, USA). Fura-2 AM, Fluo-4 AM, Alexa Fluor 568 conjugated goat anti-rabbit IgG...