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The antibodies used in this study were: anti-TACC3 rabbit monoclonal antibody (mAb) (Cell Signaling Technology, Danvers, MA, USA; 8069), anti-TACC3 rabbit polyclonal antibody (pAb) (Santa Cruz, Dallas, TX, USA; sc-22773), anti-cIAP1 goat pAb (R&D systems, Minneapolis, MN, USA; AF...
(Thr172), anti-AMPKα rabbit monoclonal antibody, anti-human S100A9 monoclonal rabbit antibody (Cell Signaling Technology, Danvers, MA, USA), anti-human IL-36γ monoclonal mouse antibody, anti-human S100A7 monoclonal mouse antibody and anti-human S100A8 monoclonal rabbit antibody (Abcam) were ...
The presence and migration of injected cells in situ were analyzed by IHC using human nuclear specific antibody (HuNu; Cell Signaling Technology, Danvers, MA, USA). Myogenic conversion of injected hAFSCs in vivo was confirmed with real-time PCR using human primers for myogenic lineage markers. ...
(Cell Signaling Technology, Danvers, MA, USA) applied at dilutions ranging from 1:50 to 1:1000 with various antigen retrieval methods and use of different amplification and detection systems, in automated instruments or manually. Tumour specimens with knownROS1rearrangement, or a cellblock of the...
Monoclonal anti-p44/42 MAP kinase, anti-Phospho-p44/42 MAPK (Thr204/Tyr202), total-Akt and phospho-Akt (Ser473) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). pFR-Luc plasmid and pFA2-Elk1 plasmid were purchased from Invitrogen (Carlsbad, CA, USA). Cell ...
(Cell Signaling Technology, Danvers, MA, http://www.cellsignal.com/), GF109203X (GFX; Tocris Bioscience), U0126 (Promega, Madison, WI, www.promega.com), (2′Z,3′E)−6-Bromoindirubin-3′-oxime (BIO; Wako Pure Chemical Industries), epidermal growth factor (EGF; R&D Systems), ...
Membranes were washed 3 times in TBS-T buffer and incubated while rotating for 1 h at room temperature with secondary HRP-conjugated antibodies (Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% milk powder solution in TBS-T buffer. For chemiluminescent detection of the HRP-...
Akt (#9272), Met (#8198), p-GSK-3β (Ser9; #9332), total GSK-3β (#9832), β-catenin (#8480), c-Myc (#5605), β-actin (#4970), and the secondary antibody anti-rabbit IgG (#7074) or anti-mouse IgG (#7076) were acquired from Cell Signaling Technology (Danvers, MA, USA...
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