necrosulfonamide (NSA), trolox (Vit E), and U0126 were purchased from Sigma-Aldrich. Captopril (CAP) was purchased from Cell Signaling Technology (Danvers, MA, USA). Z-VAD-FMK was purchased from Enzo Life Sciences
Japan). Anti-B-cell lymphoma 2 (BCL-2) was obtained from Abcam (Cambridge, UK), whereas primary antibodies against the following proteins were purchased from Cell Signaling Technology (Danvers, USA): phosphoprotein kinase B (p-PKB/AKT), total-AKT (t-AKT), p-forkhead...
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The presence and migration of injected cells in situ were analyzed by IHC using human nuclear specific antibody (HuNu; Cell Signaling Technology, Danvers, MA, USA). Myogenic conversion of injected hAFSCs in vivo was confirmed with real-time PCR using human primers for myogenic lineage markers. ...
Monoclonal anti-p44/42 MAP kinase, anti-Phospho-p44/42 MAPK (Thr204/Tyr202), total-Akt and phospho-Akt (Ser473) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). pFR-Luc plasmid and pFA2-Elk1 plasmid were purchased from Invitrogen (Carlsbad, CA, USA). Cell ...
These results are based on the use of the ROS1 (D4D6) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA) applied at dilutions ranging from 1:50 to 1:1000 with various antigen retrieval methods and use of different amplification and detection systems, in automated ...
(Cell Signaling Technology, Danvers, MA, http://www.cellsignal.com/), GF109203X (GFX; Tocris Bioscience), U0126 (Promega, Madison, WI, www.promega.com), (2′Z,3′E)−6-Bromoindirubin-3′-oxime (BIO; Wako Pure Chemical Industries), epidermal growth factor (EGF; R&D Systems), ...
Our Company Cell Signaling Technology (CST) is dedicated to providing the world’s highest quality, innovative research products to accelerate biological understanding. A privately-owned company headquartered in Danvers, Massachusetts, USA. Founded in 1999, CST employs over 600 people worldwide....
For in vivo evaluation of SIRT7-promoter binding by C/EBPs the SimpleChIP® Enzymatic Chromatin IP Kit with magnetic beads (Cell Signaling Technology, Danvers, USA) was used according to manufacturer’s instructions. Antibodies for immunoprecipitation are described in Supplementary Table 3. Quantific...
Antibodies against the following proteins were purchased from Cell Signaling Technology (Danvers, MA, USA): BAX (1:1000), cleaved-Caspase3 (C-Caspase, 1:1000), total Caspase3 (T-Caspase3, 1:1000), total AKT (T-AKT, 1:1000), phosphorylated AKT (P-AKT, 1:1000), T-mTOR (1:1000),...