Our Company Cell Signaling Technology (CST) is dedicated to providing the world’s highest quality, innovative research products to accelerate biological understanding. A privately-owned company headquartered in Danvers, Massachusetts, USA. Founded in 1999, CST employs over 600 people worldwide....
Cell Signaling Technology, Inc. Global Headquarters USA 3 Trask Lane Danvers, MA, 01923 United States Phone:978.867.2300 Fax:978.867.2400 Hours:9:00 AM - 5:00 PM (EST) Production Headquarters USA 32 Tozer Road Beverly, MA, 01915
necrosulfonamide (NSA), trolox (Vit E), and U0126 were purchased from Sigma-Aldrich. Captopril (CAP) was purchased from Cell Signaling Technology (Danvers, MA, USA). Z-VAD-FMK was purchased from Enzo Life Sciences
Immunohistochemistry analysis was conducted as described earlier [26]. Tissue sections were incubated with rabbit monoclonal antibodies against P130cas and YAP (1: 50; Cell Signaling Technology, Danvers, MA, USA). P130cas and YAP staining intensity were scored as 0, 1, 2, and 3, corresponding...
The primary antibodies against Sp1, MMP-9, Cyclin D1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Protein levels were normalized to total GAPDH or β-actin (Bioworld Technology, MN, USA). Fold-changes were determined. 2.5. Immunofluorescence assay Immunofluorescence of ...
USA) for pulldown reactions. The beads were then washed with lysis buffer and finally eluted using lysis buffer followed by Western blot analysis. The antibodies used are as follows: anti-BRD4, anti-PD-L1, anti-RelB, anti-NF-κB, anti-c-Myc (Cell Signaling Technology; CST, Danvers, MA...
Rabbit anti-Akt (1:1000) (#92725), rabbit anti-p-Akt (1:1000) (#4060), and rabbit anti-PI3K (1:500) (#4292) antibodies were all purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-SGK3 (1:1000) (bs-6475R) antibody was purchased from Bioss (Beijing, China...
against human p53, SF3B2, SF3B3, RBM17, Lamin-B1 and GAPDH were purchased from Proteintech Group (IL, USA). SCAP was purchased from Cell Signaling Technology (Danvers, MA 01,923, USA). CDK2/4/6, Cyclin D1, SF3A3, and p53 S46 were purchased from Abcam (Cambridge, MA, USA)....
(FAK), IRS-1 total, pS362/365 and pY612, AS160 pT642, MYPT1 pT696, YAP pS127, Akt total and pS473 and pT308, were from Cell Signaling Technologies (Danvers, USA), agarose-conjugated IQGAP1 antibody and, cofilin-1 total and pS3 antibodies were from Santa Cruz (Dallas, USA). Flu...
The following primary antibodies were used at indicated dilution: rabbit monoclonal to phospho-RIPK1 (Ser166, clone D1L3S) diluted 1:1 000 (65746, Cell Signaling Technology, Danvers, MA, USA); rabbit polyclonal to PARP1 diluted 1:1 000 (ab137653, Abcam); mouse monoclonal to SQSTM1/p62...