Pan-cancer comparison of expression profiles We compared RNA-seq profiles from 8282 primary tumors from TCGA with 666 cell lines from CCLE across 22 overlapping tumor types (Supplementary Data1,2). Primary tumors were used in all tumor types except for SKCM, in which case the metastatic tumors...
Over‐expression of secreted proteins from mammalian cell lines. Protein Science. 2014; 28: 517–525. doi:10.1002/pro.2439 139. Butler M. Animal cell cultures: recent achievements and perspective in the production of biopharmaceuticals. Applied Microbiology and Biotechnology. 2005; 68: 283–291. ...
1.SingleR comes with five packages humanhpca.se<-HumanPrimaryCellAtlasData() bpe.se <-BlueprintEncodeData() DICE<-DatabaseImmuneCellExpressionData() NHD<-NovershternHematopoieticData() MID<-MonacoImmuneData() mouse MRD<-MouseRNAseqData() IGD<-ImmGenData() 2.determine how many marker genes nee...
3f). Single-cell RT–qPCR data showed no expression of the SGLT2 transcript Slc5a2 in gut epithelial cells (Fig. 3b). As expected, the SGLT inhibitor phloridzin did not affect the response to luminal sucralose (N ≥ 5 mice; not significant compared to preinhibition response; Fig. ...
1,25(OH)2D3-mediated cell death in glioma cells is dependent on protein synthesis and upregulation of c-myc expression, IL-6, vaso-endothelial growth factor and gadd45 gene expression [117]. In glioblastoma multiforme cell lines that were resistant to 1,25(OH)2D3, resistance cannot be ...
These findings confirmed the yeast data (Figure 3B) and indicate that the rab1 bind- ing domain of Iporin needs the whole RUN motif. Tissue-specific expression and cellular localization of Iporin Northern blot analysis of various human tissues indi- cated, that Iporin is ubiquitously expressed...
Data collection and preprocessing The bulk transcriptome data and corresponding clinical data of BC patients receiving NAC were obtained from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/) via GSE25055, GSE25065 and GSE22226. We chose GSE25055 with the most BC...
Value of the data 1. Data Table 1 showed data on the global gene expression profile in MCF-7 and MDA-MB-231 cell lines treated with vehicle (DMSO) or DS in vitro. Table 2, Table 3, Table 4 showed gene ontology analysis based on molecular functions (Table 2), biological processes (Ta...
cell lines as well as RCC xenografts through suppression of survivin levels [31]. These authors demonstrated that the synergistic effect of temsirolimus with vorinostat on cell viability, colonogenic survival inhibition and apoptosis induction was due to the synergistic inhibition of survivin expression ...
We compared genes that were always expressed in SH-SY5Y across 247 different conditions (Additional file5) from the GEO (Gene Expression Omnibus) database [42] to those that were never expressed, whenever the corresponding probe was present. Here, the threshold used to determine whether genes we...