The Cell Ranger pipeline version 2.1.1 was used with the mm10 mouse reference genome version 2.1.0 to convert base calls to fastq format and align, map and count genes. Analysis of nodose single-cell sequencing data RStudio package Seurat version 3.1.0 was used for the analysis59. We ...
Cell lines BHK and HeLa cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum and 1% glutamine at 37°C in a humidified 5% CO2 incubator. Cytosol/membrane fractionation HeLa cells were grown on 10 cm tissue culture plates and transiently ...
cell lines and 16S rRNA sequences from the NCBI nt database was performed using Muscle56 with default parameters. The phylogenetic tree was constructed by the UPGMA method57 with MEGA X software58. Branch supports were tested by bootstrap resampling (1,000 replications). Preparation of cells ...
Several large studies have been publicly released testing candidate molecules, often with corresponding molecular profiles of the cell lines used for drug screening. These studies have become invaluable resources for the research community, allowing researchers to leverage the collected data to support ...
cell lines increased for all but neuroblastoma (Table4). Note that in the case of neuroblastoma, the main actors which contributed to the high BC-ratio computed on the real data, already were OMIM genes, damaged in the cell line. Therefore a lower BC-ratio for neuroblastoma was expected in...
(Supplemental TableS2), Gurova et al. proposed that p53 signaling in RCC might be repressed by some other mechanism. They found that all four RCC-derived cell lines (RCC26b, RCC45, RCC54, and RCC72) tested maintained wild-type p53. However, these cell lines were not capable of trans...
We will create a view over the base table that will, in essence, replace the table in the eyes of the end user or application. Here is what the view definition should look like.Note: Some parts of the code snippet presented in the following table have been displayed in multiple lines ...
We will create a view over the base table that will, in essence, replace the table in the eyes of the end user or application. Here is what the view definition should look like.Note: Some parts of the code snippet presented in the following table have been displayed in multiple lines ...
Use the following format: Database: 12345 (e.g. TAIR: AT1G01020; CCDC: 734053; PDB: 1XFN). Learn more about linking research data and research articles in ScienceDirect. Research Elements This journal enables the publication of research objects (e.g. data, methods, protocols, ...
a DNA analysis of a bone fragment more than 50,000 years ago proved it a hybrid between Neanderthal and Denisovan; proteins within a cell worked by condensing into separated liquid droplets; a DNA database comparison helped police nab cold killers; the first gene-silencing drugs got approved. ...