Ectopic expression of ZNF471 significantly inhibited cell proliferation resulting in a delay in cell doubling time (P<0.05). The cell doubling analysis was performed using the doubling time calculator (http://www.doubling-time.com/compute.php). d ZNF471 inhibits anchorage-dependent tumor growth in...
Variance component analysis demonstrated that variations in cell viability were primarily associated with the choice of pharmaceutical drug and cell line, and less likely to be due to the type of growth medium or assay incubation time. Furthermore, careful consideration should be given to different ...
Though to a lesser extent, growth medium (type of medium/addition of FBS and medium volume) and resazurin incubation time also had an impact on cell viability (P < 0.05; Fig. 4). These findings highlight the importance of assay optimiza- tion for each cell line to...
The doubling time (DT) of carcinomas was calculated when≥ 2 CT scans were available. Using an online DT calculator for growth rate of a lesion or a mass [26], the diameter and date of the first and second examinations were entered, and the DT in days was generated as described prev...
requiring serial passaging to presenescence and senescence, VSMCs in the proliferative phase (P6–P9) were used.36 Nesprin-2 siRNA oligomers targeting the nesprin-2βΔKASH1 variant have been described previously.10 PDT was determined using the online calculator at www.doubling-time.com/compute...
the in vivo dataset (Spearmanρ = 0.10,P-value = 0.03 and Spearmanρ = −0.12,P-value = 0.01, respectively), suggesting that the in vivo dataset might not have enough infected cells, and thus power, to identify significant correlations. In line with this, lncRNAs ...
An advanced HSDPA+ network can theoretically support up to 28 Mbit/s and 42 Mbit/s with a single 5 MHz carrier. Doubling the bandwidth to 10MHz results in theoretically, double the sustained data rate. Related terms: HSDPA (High-Speed Downlink Packet Access) HSUPA (High-Speed Uplink Packet...
Fitting procedures and response metrics have been described in detail previously (Hafner et al., 2016, Hafner et al., 2017b) (Figure S1A), and all routines and data can be accessed on-line or via download at http://www.grcalculator.org/. We found that response curves for technical ...
An online calculator was used at: http://www.chestx-ray.com/index.php/calculators/doubling-time, by analyzing the difference between tumor volumes the day before starting each treatment and at the end of the experiment. e Cell extracts derived from T1 tumors treated with vehicle (1) or ...
The cells were then resuspended in fresh ice cold 1× PBS at 0.5 million cells/mL. A Bio Rad cell sorter (Supplementary Table 4) was used to gate single cells, followed by removal of doublets from the population. The FL-2 laser line was used to detect the PI, and a histogram was...