Cell doubling per 24 hours was determined based on cell numbers comparing cells seeded and on day 6 using the following formula: duration (in days) x log(2)/log(final cell number) – log(initial cell number). Melanoma-TIL co-culture experiments Melanoma cell line 2686 was pre-treated with...
The doubling time was calculated from the logarithmic growth curve using the following formula: v = lgN − lgN0/lg2 (t − t0), with doubling time = 1/v, where N = number of cells and t = time. Morphology and immunocytochemistry Growth pattern and cell ...
Cell doubling times were calculated using the formula DT = logN/log2 (h) in which N, median number of cells per clone and h, age of clone; the rate of increase in cell number is 1/DT. Acknowledgements We are very grateful to Martin Heisenberg, Dieter Maier, Anette Preiss, Jim Posa...
The doubling time for the cells was calculated using the following formula: dt = t × ln2/ln(Ct/Co) (1) where dt is the doubling time, t is the time between cell counts Ct and Co, Co is the initial count, Ct is the count after time t, and ln is natural log. The doubling ...
“HCC2”. These primary HCC cells are proliferative. The cell doubling time is about 2.5 days for HCC1 cells and 4 days for HCC2 cells. Human primary adult hepatocytes, purchased from the Cell Bank of Fudan University (Shanghai, China), were derived from the liver of a partial ...
By equalizing growth energy of this sample to the energy of the induced inhibition to [~(14)C] thymidine incorporation, CGE gained due to cell cycle arrest was 4862 MeV =0.21 Emad. According to Emad formula the corresponding cell doubling time is 1.12 times that of cells at the stage of ...
This might be because the cells in the high-uptake S/G2/M phase progresses to the G1/S phase, judged from the doubling time (i.e., 45.7 ± 3.2 h44). In vivo, the BPA concentration peaked 2–3 h after intravenous injection22. We selected 4 h after administration when ...
Cell doubling time was calculated and graphs were plotted using GraphPad Prizm software, ver. 5.02. Wound Healing Assay 3× 105 cells were seeded on a 6-well plate and 24 hours later "wounds" were scratched with a 1000-mcl pipette tip, washed with medium and photographed with a digital ...
Importantly, Pin1 knockdown led to significant decrease in cell proliferation, as shown by cell doubling time (Figure 6b) as well as FCM analysis (Figures 6c and d); simultaneous knockdown of WWP1 largely rescued proliferation defect induced by knockdown of Pin1 (Figures 6b–d). Furthermore,...
The doubling time was calculated following the formula: DT=48*[lg2/(lgNt(number of cells at day4)−lgNo (number of cells at day2))]. RNA Seq and Data Analysis Total RNA was isolated from primed hES cells, hEPS cells and naïve NSHM-hES cells, mES cells and mEPS cells using the...