The fundamental integral equation governing the growth of a cell culture is solved for the cell doubling-time distribution with the use of Laplace transform methods. Both analytic and numerical solutions are discussed. The statistical moments of the doubling-time distribution are obtained without inverti...
Cell population doubling time Cells were plated into 6-well plates (1 × 104 cells/well) and cultured at 37°C. Cell population doubling time (PDT) was calculated using the following equation: PDT (hr) = (log2 × t)/(logNt - logN0), where t = time in culture (hr), Nt = final...
Cell population doubling time calculation and cell cycle assay were performed as previously described [11]. Cells were seeded into 6-well plates (1 × 104cells/well) and cultured at 37°C. Cell population doubling time (PDT) was calculated using the following equation: PDT (hr) = (log...
The net growth rates and the doubling times were determined using the following equation: Netgrowthrate(μnet)=In(Nf)−In(Ni)/△t Doublingtime(Td)=In(2)/μnet where Ni and Nf are the total cell count immediately before and at the end of a given stage, respectively, and Δt is ...
In the diluted media the cells have small volumes and the doubling time is increased. When the cell volume increase per time per cell in a given medium is expressed as a function of the cell volume in this same medium, a direct proportionality is found. From this equation the minimum cell...
Simplifying the above equation and approximating A=2rL. Here A is the cell area output from BACMMAN.ρ=24L(L2+A)A(3L2−A) Elongation rate The instantaneous elongation rate was calculated based on the log-difference in cell length between consecutive frames as log(Lt)−log(Lt−Δt)Δ...
We now consider the inference of microbial growth rates (strictly, we infer the specific growth rate: the time derivative of the logarithm of the population size). The population size as a function of time is commonly fit to a parametric equation2, although these equations are restrictive and ...
Average cell doubling times and SDs, which were calculated using nonlinear regression-Gaussian equation (GraphPad Prism 6), are shown. We then determined whether Cell-Double times differed among Groups a–f, which had different levels of reproductive ability (Fig. 3d–i and Supplementary Fig. ...
Therefore, the effect of cell inhibition can be added the same way as the Logistic equation as it reflects the concentration of free space in terms of the (imaginary) cells (available): (11.78)μG=μmaxSKS+S1−XX∞ where X∞ is the maximum cell concentration or the packing density of ...
First, a ∆SGT value was calculated for each sample according to the following equation: ΔSGT = (SGTTreated − SGTNormalizer) where the SGT of untreated normalizer cells was subtracted from the SGT of treated cells. Second, a ∆∆SGT value was calculated by subtracting the ...