Cell population doubling time calculation and cell cycle assay were performed as previously described [11]. Cells were seeded into 6-well plates (1 × 104cells/well) and cultured at 37°C. Cell population doubling time (PDT) was calculated using the following equation: PDT (hr) = (log...
Doubling Time Calculation The cells were removed from the plates using 0.05% trypsin-EDTA (Invitrogen): they were counted and plated onto 24-well plates that were pre-seeded with feeder cells at a density of 10,000 cells per well in the appropriate medium without Y27632. The growth rate was...
We discuss the validity of the two formulas currently used for the calculation of T-pot one based on LI and the other on the nu-function, in conditions of non-uniform cell loss, Moreover, we propose two formulas for the estimation of the cycle time T-c, which require, in addition to...
Fig. 3: Calculation of plasmid copy number and promoter activity across different strains. The dashed lines in all graphs are the distributions for E. coli NEB 10-beta. a Plasmid copy number (n = 1449, p-values from the two-sample Kolmogorov–Smirnov test for pooling the replicates are...
where Nt is the cell number at time t, N0 is the initial number of cells, t is the culture time, and gr is the cell growth rate. The calculation via the curve-fitting technique revealed that the estimated gr values were 0.040, 0.040, 0.041, and 0.048 per hour for the muscle cells ...
Calculation of Doubling Time and Doubling Time Ratio Doubling time (DT) is the period of time required for a cell to double in number. DT of MSC and DT ratio were calculated as follows. Number of MSC doubling = log 2 ( N N 0 ) . N is the numb...
After the validation using CSSC(GMP), our QC testing and SI calculation proved to be suitable for evaluating the potency of cell products in a scar-reducing corneal wound healing. Cell therapies are actively being pursued in the hope of treating a broad range of conditions and intractable ...
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Cell cycle (doubling time) was defined as the time from one to the next cytokinesis. Astrocytes with EGFP fluorescence were analyzed, and cells with tdTomato were considered to be RAC1-KO astrocytes. Experiments were performed in duplicate, and at least three independent transfection experiments ...
Calculation of BPA concentration from the etch pits To obtain the BPA concentration within the cells, PHITS simulations were performed at the same experimental condition (i.e., the same BPA concentration as administered in the experiments), and the frequency of 10B(n,α)7Li reactions that occurr...