ZNF471 overexpression reduced cell proliferation by increasing the cell doubling time (SiHa-ZNF471, 48.52 h; CaSki-ZNF471, 72 h) compared with control cells (SiHa-Vector, 21.04 h; CaSki-Vector, 30.63) (P<0.05; Fig. 3c). The anchorage-dependent assay showed the tumor growth suppressive ...
1B). The doubling time was estimated to be 26 h (Fig. 1C). Figure 1 MaS-3 shows a distinct morphology. (A) Cells growing out of tumour fragment 4 days after seeding. (B) Morphology of MaS-3 cells passage 10 under phase contrast microscope (a) ×5, (b) ×10, (c) ×20. (...
The timing of primary proliferative effect seem to stay in correlation with RCC cells doubling time, that is 45 h for 786–0 cell line (Williams et al. 1978), and 32 h for ACHN, 36 h for Caki-1 cells, and 24–30 h for HEK293 cells (Cowley et al. 2014). The activating insulin...
From the growth curve, we estimate the maximum growth rate as the maximum time derivative of the logarithm of the growth curve2: where we denote the growth curve as y(t). The doubling time is ln(2) times the inverse of the growth rate. We define the lag time as the intercept of the...
Reactions were run on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Cat#:1855485). Gene expression was normalized to GAPDH. Microsoft excel was used to calculate standard deviations for the error bars. Significance of difference between cohorts and conditions was determined by an ...
Moreover, cytoplasmic division occurs in the late stage of mitosis to physically separate a parent cell into two daughter cells, doubling the cell count. Therefore, the increased doubling time in the KO strain may be due to impaired cytokinesis. Thus, the growth defect displayed by the KO ...
Statistical analysis was performed using the 2−ΔΔCT method in Excel and two-way ANOVA or unpaired t test. P values: * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001, *** ≤ 0.0001. Full size image There are 30 genes in the apicoplast genome, which mostly encode ribosomal...
Standard error of the mean was calculated for all tissue sections found to contain cells (Microsoft Excel version 2003). Transgene methylation analysis by bisulfite sequencing Bisulfite treatment of DNA followed by sequencing was used to determine the endogenous transgene promoter pattern of methylation ...
The doubling time was calculated by assuming exponential growth in this time period. Reporting summary Further information on research design is available in the Nature Research Reporting Summary linked to this article.Data availability Source data are provided with this paper. Genetic part sequences ...
Ki-67 in screen-detected, low-grade, low-stage prostate cancer, relation to prostate-specific antigen doubling time, Gleason score and prostate-specific antigen relapse after radical prostatectomy. Scand J Urol Nephrol 2009; 43: 12–18. 13. Kelly K, Cochran BH, Stiles CD, Leder P. Cell-...