Extracellular matrix protein 1 (ECM1) was identified as a DEG downregulated in LC for the modification of HF-MSCs. ECM1 is a secreted glycoprotein and is involved in embryonic chondrogenesis, skin differentiation, angiogenesis, and cell proliferation [23,24,25,26,27]. ECM1 is mainly secreted ...
+cells and CD97+cells under shear stress conditions can lead to extraction of the CD97 NTF16,17. CD97 and CD55 deficiency are associated with mild granulocytosis and reductions in spleen type 2 conventional dendritic cells17,18,19,20. However, the cellular changes caused by CD55 engagement ...
Comparing the proteome of a given type of cells, which under s-µg either grew adherently or within an aggregate suggests that amongst other cell components, protein complexes are affected by annulling gravity, which play a role in cell-cell and cell-ECM (extracellular matrix) contacts [4]...
3E). GSEA revealed that trajectory 1 was mainly associated with “External encapsulating structure” and ECM organization. Trajectory 2 was related to “locomotion” and “development”. Trajectory 3, the “CDC-trajectory”, was linked to “response to cytokine”, immune system processes and ...
Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue Engineering Interactions with the extracellular matrix (ECM) play an important role in regulating cell function. Cells cultured in, or on, three-dimensional ECM recapi... S Uriel,E Labay,M Francis-Sedlak,... - 《Tissu...
Tumor microenvironment (TME) of CRC is composed of extracellular matrix (ECM) and cellular components including immune cells, tumor endothelial cells, tumor associated fibroblast and tumor cells. The interaction of cellular and non-cellular elements through various signaling pathways is another important ...
In the mouse melanoma model, 3 × 104 B16.F10 tumour cells were injected in 50 µL RPMI with ECM (1:2) subcutaneously in the dorsal region of female 10-18 weeks old C57BL/6J mice (purchased from Charles River). The B16 tumour experiments were performed according to the regulations of...
When the genes (q < 0.001) are subjected to a KEGG pathway database to obtain a similar module analysis using the DAVID 2008 web-based tool, the top-ranked canonical pathways in the NGMGCT group again include cell motility (such as Focal adhesion, ECM-receptor interaction and Gap junction)...
(ECM)-modifying proteins after co-culture with both PDAC subtypes (Fig.3e). In addition to many cytokines, interaction with cancer cells broadly induced ECM regulators such as matrix metalloproteinases (MMPs), a disintegrin and metalloproteinases (ADAMs), and ADAMs with thrombospondin motifs (ADAM...
Genomic DNA and RNA extraction Tumor tissues and corresponding normal mucosae were obtained from surgically resected specimens, and after a piece was removed for fresh single-cell prep the remaining sample was snap-frozen in liquid nitrogen and stored at −80 °C. Before bulk RNA/DNA extracti...