In spite of recent progress, there are still knowledge gaps on how the different lung cell types function during regeneration and whether there are any unknown cell types. In this study, we used scRNA-seq to analyze the consequences of epithelial cell type-specific depletion through inducible expr...
Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue Engineering Interactions with the extracellular matrix (ECM) play an important role in regulating cell function. Cells cultured in, or on, three-dimensional ECM recapi... S Uriel,E Labay,M Francis-Sedlak,... - 《Tissu...
6a,b). Importantly, the concomitant upregulation of ECM genes (COL1A2, COL3A1, FN1 and POSTN) suggested the onset of a fibrotic response, which was corroborated by abundant ECM deposition in the subepicardial space and the emergence of α-smooth muscle actin-positive (α-SMA+) ...
those highly-expressed genes were mainly involved in focal adhesion, regulation of actin cytoskeleton, ECM-receptor interaction, and hippo signaling pathway (Fig.3D). Thus, SC3 may be related to the maintenance of basic metabolism and anatomical structure of the periosteum. ...
Comparing the proteome of a given type of cells, which under s-µg either grew adherently or within an aggregate suggests that amongst other cell components, protein complexes are affected by annulling gravity, which play a role in cell-cell and cell-ECM (extracellular matrix) contacts [4]...
and ecm organization. trajectory 2 was related to "locomotion" and "development". trajectory 3, the "cdc-trajectory", was linked to "response to cytokine", immune system processes and secretion (fig. 3 d, suppl fig. s7e). to further reveal whether cdcs constitute a transcriptional transition...
When the genes (q < 0.001) are subjected to a KEGG pathway database to obtain a similar module analysis using the DAVID 2008 web-based tool, the top-ranked canonical pathways in the NGMGCT group again include cell motility (such as Focal adhesion, ECM-receptor interaction and Gap junction)...
No matter ECM or cytokines, they are all secretory proteins [12]. Secretome analysis based on LC-MS is a powerful method used to identify and quantify the secretory proteins in cell culture supernatants. So, we further performed secretion profile in this study. The data showed that NMN ...
such as context-specific secretion of cancer cell-derived EMT-promoting molecules and immunomodulators that correlated with differential immune cell recruitment in vivo, as well as distinct qualitative and quantitative contributions of cancer cell-derived proteins to the tumor extracellular matrix (ECM). ...
Genomic DNA and RNA extraction Tumor tissues and corresponding normal mucosae were obtained from surgically resected specimens, and after a piece was removed for fresh single-cell prep the remaining sample was snap-frozen in liquid nitrogen and stored at −80 °C. Before bulk RNA/DNA extracti...