进一步对差异基因GO分析,NADH脱氢酶活性途径在IDA处理的细胞中特异性富集,这意味着IDA-AHR轴可能通过NADH介导的辅酶Q10的还原促进FSP1调控的抗铁死亡。通过RT-qPCR和western blotting实验证实在IDA处理下,ALDH1A3的mRNA和蛋白水平显著升高(图5b、c),而在其他色氨酸代谢物处理下,ALDH1A3没有显著变化,这表明ALDH1A3可...
Blotting, Far-WesternProtein Interaction MappingIndicators and ReagentsIsotope LabelingThe parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with...
Single-Cell Western BlottingProtocol pp 455–464 Cite this protocol Western Blotting Syed M. S. Quadri Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312)) 10k Accesses 2 Citations 1 Altmetric Abstract Cell heterogeneity is a variation in cellular processes in ...
Single-Cell Western BlottingProtocol pp 1–9 Cite this protocol Single Cell Protein Analysis Elly Sinkala & Amy E. Herr Part of the book series: Methods in Molecular Biology ((MIMB,volume 1346)) 3385 Accesses Abstract Little headway has been made in single cell protein analysis, aside ...
Western blotting Both cells and culture supernatants were harvested for western blotting. After washing, cell sediments were lysed in RIPA lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS) with cocktail, and sonicated. The total protein ...
Whole cell lysates were collected after 5 min incubation for Western blotting. Immunoprecipitation and immunoblotting were per- formed as described in MATERIALS AND METHODS. The lower panels show the intensity of phosphorylated AMP kinase (p-AMPK) that was measured using the NIH Image Program and ...
Nature Cell Biology volume 26, pages 825–838 (2024)Cite this article 12k Accesses 8 Citations 38 Altmetric Metrics details Abstract Blocking the import of nutrients essential for cancer cell proliferation represents a therapeutic opportunity, but it is unclear which transporters to target. Here we ...
53) followed by western blotting against RPB1. As recently reported42, we observed RPB1 polyubiquitylation after formaldehyde treatment in G1-synchronized RPE1 cells (Fig. 3b); this was partially CSB dependent (Fig. 3c). On treating S-phase RPE1 cells with 5-aza-dC, however, only weakly...
Western blotting and immunoprecipitation Cell lysates were isolated using lysis buffer (20 mM Tris, pH 7.5, 100 mM NaCl, 1 mM EDTA, 2 mM EGTA, 50 mM β-glycerophosphate, 50 mM NaF, 1 mM sodium vanadate, 2 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride (PMSF),...
purchased from Cell Signalling Technology (Bax Antibody #2772), suited solely for immunoblotting applications according to the manufacturer’s information, clearly indicated loss of Bax expression in the respective Bax deficient cells (Fig.1A). We also failed to detect a loss in the supposed Bax ...