Western blotting Inhibitor treatment Histology Cell culture Related Standards NGSS: Out of Scope AP: Unit 4: Cell Communication and Cell Cycle LB: 6.6: Hormones, Homeostasis and Reproduction Learn More About This Simulation In this simulation, you will learn how tumor cells send signals to surroundi...
How to Conduct A Co-IP 1. Sample Preparation The first step in the Co-IP protocol is to prepare the sample to be analyzed, which can be derived from cells, tissues, or other biological sources. Proper sample preparation is critical to ensure efficient immunoprecipitation and accurate detection...
2b). Using a probe identical to the one spotted on the cDNA microarray, we performed a Northern blot analysis. The blot demonstrated the up-regulation of the perforin transcript in leukemia LGL cells (Fig. 2c), but the fold increase was neither 103 as indicated by oligonucleotide array nor...
2I). To prepare the fibrils, we followed the protocol described in Kumar et al., 2020. In brief, the lyophilized α-syn was dissolved in PBS to a final concentration of ~300 μM and incubated with shaking at 37 °C for five days at 1000 rpm. Next, we used our filtration protocol ...
human beings These examinations also include procedures to determine, measure, or otherwise describe the presence or absence of various substances or microorganisms. Facilities which only collect or prepare specimens, or act as a mailing or distribution center, are not considered to be laboratories, al...
human beings These examinations also include procedures to determine, measure, or otherwise describe the presence or absence of various substances or microorganisms. Facilities which only collect or prepare specimens, or act as a mailing or distribution center, are not considered to be laboratories, al...
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human beings These examinations also include procedures todetermine, measure, or otherwise describe the presence or absence of varioussubstances or microorganisms. Facilities which only collect or prepare specimens,or act as a mailing or distribution center, are not considered to belaboratories, although...