对于药物筛选,PSC来源的类器官由于个体间差异大、传代能力差而难以应用于大规模药物筛选(图7E-G),而突变FeBOs可通过传代产生大规模等同的类器官株。研究者建立TP53, PTEN, NF1三敲FeBOs以模拟胶质母细胞瘤并进行突变-药物敏感性实验(mutation-drug sensitivit...
Assay of sensitivity of cancer cell to anticancer drug in glass capillaries. Chin J Cancer Res. 1992; 4 :40–45.He Z M, Wei L Y, Peng X (1992). Assay of sensitivity of cancer cell to anticancer drugs in glass capillaries. Chin J Cancer Res 4, 40-45.He Z M,Wei L Y,Peng X....
The development of reliable, accessible, and scalable viability assays based on cell death–associated effects of biomarkers is extremely important for efficient screening of drug toxicity. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay is currently one of the ...
A total of 83 specimens of surgically resected tumours from 78 patients with oesophageal cancer were assayed for drug sensitivity using an adhesive tumour cell culture system (LifeTrac CSA assay). Seventyone of 83 specimens had a sufficient number of cells to permit growth in culture and 57 of...
We ascertained that one center had used the CellTiter-Glo ATP-based assay and a luminescence plate reader as a proxy for counting the number of viable cells in a microscope. CellTiter-Glo is among the most commonly used assays for measuring cell viability and was therefore a logical substitute...
6. Gocho Y, Liu J, Hu J, et al. Network-based systems pharmacology reveals heterogeneity in LCK and BCL2 signaling and therapeutic sensitivity of T-cell acute lymphoblastic leukemia.Nat Cancer. Mar 2021;2(3):284-299....
Finally, a drug was considered specific for a subpopulation if its FDR was less than 0.1 and the median enrichment score across the other subpopulations was greater than zero. This analysis has been implemented in the runDiffDrugAnalysis function of the DREEP package. Drug combinations assay 4...
Data regarding assay performance (specificity, sensitivity, and reliability) should be submitted to the FDA as part of the validation process. Manufacturing and Characterization Issues for Stem Cell Products This section describes in detail, the various recommendations currently used by FDA reviewers to ...
The role of Apigenin and its combination with SAHA in DNMT inhibition was investigated by performing DNMT enzymatic kit-based assay. Apigenin inhibited DNMT activity in a dose-dependent manner (Fig.13A). Nearly 70% of DNMT inhibition was observed at IC50concentration, while total DNMT inhibition ...
Most drug treatments were performed in pairs with a single untreated control, resulting in 20 distinct treatment conditions and 12 independent untreated controls. Average population expansions between t1 and t2 with each condition based on A600 measurements are listed in Supplementary Data 1....