对于药物筛选,PSC来源的类器官由于个体间差异大、传代能力差而难以应用于大规模药物筛选(图7E-G),而突变FeBOs可通过传代产生大规模等同的类器官株。研究者建立TP53, PTEN, NF1三敲FeBOs以模拟胶质母细胞瘤并进行突变-药物敏感性实验(mutation-drug sensitivit...
Table 5. Immunocompetent in vitro skin models Skin modelSpecificationsReadout parameterLimitationsReferences Nott-Sheff 3-D K-F-DC Coculture of dendritic cells, within an agarose gel, alongside primary fibroblasts and keratinocytes Histology, cytotoxicity assessment assay (MTT/MTS), cytokine release and...
The in vitro adventitious virus assay can sometimes be used to identify certain viruses. The in vivo and in vitro virus tests detect complimentary virus types (Table 73-1). TABLE 73-1. Tests to Detect Adventitious Viruses In Vivo Adventitious Virus TestingIn Vitro Adventitious Virus Testing ...
Thus, the applicability of different methods described above to the different areas of cell death investigation are summarized in Table 1 and discussed below. Table 1 Advantages and disadvantages of methods used for drug cytotoxicity analysis. Full size table Formazan-based assays (MTT, MTS, XTT, ...
Interestingly, a link could be observed between rank orders of the CRC cell lines (Table 1) and GI50 values (Figure 6B), suggesting that these new signatures might be worth pursuing for assessing chemo-sensitivity. Discussion Selection criteria for cell-based models are often ill defined and ...
Intra-tumour heterogeneity (ITH) presents a significant obstacle in formulating effective treatment strategies in clinical practice. Single-cell RNA sequencing (scRNA-seq) has evolved as a powerful instrument for probing ITH at the transcriptional level,
(1B1, 2B1) and AF405 may be applied in large scale drug screening studies (Table2). Experiments using CB or AF405 as probe substrates demonstrate that these dyes are suitable to detect OATP substrate/inhibitor interactions (Fig.5). The IC50values obtained in our assay are in good agreement...
Takes the sensitivity data from a PharmacoSet, and summarises them into a drug vs cell line tablepSet
We ascertained that one center had used the CellTiter-Glo ATP-based assay and a luminescence plate reader as a proxy for counting the number of viable cells in a microscope. CellTiter-Glo is among the most commonly used assays for measuring cell viability and was therefore a logical substitute...
In comparison to published assays (Table 1), our assay has excellent performance based on its sample input and screen throughput. Moreover, given that input cell concentration and loading volume could be adjusted, there is much flexibility for the number of assay conditions to be screened on ...