ResazurinResorufinThe increasing applications of quantum dots (QDs) as optic tools in life science have stimulated researchers to evaluate the effects of these nanoprobes in cell viability using a variety of methods, especially colorimetric ones. One of the most applied tests is the MTT assay. In ...
CellTiter-Blue® Cell Viability Assay(Cell Titer-Blue® 细胞活力检测) 提供了一种均质、荧光的方法来检测细胞活力。检测原理是基于活细胞能将一种氧化还原染料( 刃天青,resazurin) 转化成一种荧光终产物( 试卤灵,resorufin)。由于不具有活力的细胞很快丧失新陈代谢能力,故而不能产生荧光信号。均质的检测方法就...
The Resazurin Cell Viability Kit is a fluorescent assay that detects cellular metabolic activity. The blue nonfluorescent resazurin reagent is reduced to highly fluorescent resorufin by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount ...
Resazurin can be utilized as a simple and quantitative method for measuring cell proliferation, viability, and cytotoxicity. It is a non-toxic, water soluble, redox-sensitive dye that changes from its blue/non-fluorescent state to a pink/highly-fluorescent state upon reduction by viable cells. Fl...
Cell Viability Assays Market by Product (MTT Assay, XTT Assay, Calcein, Resazurin, Flow Cytometry, Spectrophotometer), Cell Type (Human, Animal, Microbial), Application (Basic Research, Stem Cell, Discovery & Development) - Global Forecast to 2023 ...
This homogeneous assay involves simply adding a single reagent to the cell culture and measuring the fluorescence intensity (excitation wavelength=530-570nm, emission wavelength=590-620nm) after an incubation step. The resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye re...
VisionBlue快速细胞增殖检测试剂盒是迄今为止最灵敏、最简便的细胞增殖和细胞活力的定量测定方法.只需简单地向细胞培养液中加入一种试剂进行孵育,然后读取荧光强度值即可实现定量检测.VisionBlue检测技术使用刃天青(resazurin)作为氧化还原染料,该染料本身并无荧光,但是当进入具有代谢活力的活细胞后,便会被还原,还原后的染...
Resazurin is a fluorescent dye for the quantitative measurement of cell proliferation, viability, and cytotoxicity. MTT Cell Proliferation/Viability Assay MTT can be used to safely assess cell proliferation, cell viability, and/or cytotoxicity.
cell viability (IC50) and the area under the dose-response curve (AUC) values, we tested several experimental parameters that may affect replicability and reproducibility (e.g. storage of the pharmaceutical drugs, cell culture conditions, and resazurin assay conditions; Table 1 a...
4.2Resazurin Overview of Cell Viability Assays ParameterAssay/ReagentsModeInstrumentPrinciple Membrane IntegrityTrypan Blue vital stainsColorimetricHemocytometerTrypan Blue,Trypan UltraBlue™,Trypan Red Plus™, andTrypan Purple™are membrane-impermeant vital stains. Live cells with uncompromised membrane integr...