A colorimetric assay based on Resazurin was developed as a time kinetic assay (KRA) suitable for bacterial concentration measurements. An optimization was performed by finding excitation and emission wavelengths for fluorescent acquisition. A comparison of two non-related bact...
货号:75005 规格:5g 价格:1002 物种:其它 宿主:其它 抗体亚型:其它 荧光染料:其它 产品介绍 技术参数 相关产品 产品介绍 Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast ...
货号:75005 规格:5g 价格:1002 物种:其它 宿主:其它 抗体亚型:其它 荧光染料:其它 产品介绍 技术参数 相关产品 产品介绍 Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast ...
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2.5 Resazurin assay The resazurin assay also known as Alamar Blue assay offers a simple, rapid, and sensitive measurement for the viability of mammalian cells and bacteria. Living cells are metabolically active and are able to reduce via mitochondrial reductase, the nonfluorescent dye resazurin to ...
(hydroresorufin), and hence an underestimation of cellular activity. This test is similar to MTT, so do the problems. The differences for Alamar Blue assay are the higher sensitivity and the non-toxic reactants.296However, it has been observed a interference of the carbon NP itself in the ...
tularensis LVS bacteria in HEK293 cells 22 h post-infection 1.PROTOCOLCell Assay 1 F. tularensis is cultured in TSBc supplemented with 44 M Resazurin sodium salt at 37C with shaking for 24h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 ...
This protocol was adapted to a microplate-based assay, using resazurin as viability indicator. The RMM was as accurate as the PCCT, had an identical detection limit and showed high intermediate precision. Using the validated RMM, it was shown that H2O2 combined with silver possessed a higher ...
2.5Resazurin assay Theresazurinassay also known as Alamar Blue assay offers a simple, rapid, and sensitive measurement for the viability of mammalian cells and bacteria. Living cells are metabolically active and are able to reduce via mitochondrial reductase, the nonfluorescent dye resazurin to the ...
Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a high...