图4 CD8+ T 细胞产生的TNF-α 和IFN-γ 的流式细胞术检测Fig 4 Fluorescence activated cell detection of TNF-α and IFN-γ produced by CD8+ T cells 2.5 Stat3 敲除对CD8+ T 细胞FasL 和GraB 表达的影响 如图5 所示,SP 中FasL 的比率在WT CD8+T 细胞与ΔStat3 CD8+T 细胞无显著差异(P=0.999);...
of HnRNP L sensitizes prostate cancer cells to activated Jurkat T cells mediated killing by downregulating the PD-L1 protein levels and promoting the activated Jurkat T cells-induced cancer cell ferroptosis, and enhances anti-PD-1 therapy efficacy by recruiting CD8+ T cells in vitro and in vivo...
CD8+ T cells purified from healthy donors were activated with anti-CD3/anti-CD28 microbeads in the absence (Tex cells) or presence (Tex cells + IFN-Is) of 1,000 U/mL human universal type I interferon. Surviving cells were passaged into fresh cultures every 48 h and were collected at da...
2. Immunotherapy based on CD8+ T cell: mechanisms and challenges 2.1. Immune checkpoint inhibitors (ICIs) ICIs block the interaction between immune checkpoints and their ligands, alleviating immune suppression and reactivating CD8+ T cells to exert anti-tumor effects [12]. Inhibitors targeting cytoto...
To test anti-tumor activity in vivo, we implanted EG7 tumor cells in congenic (CD45.1+) mice followed by adoptive transfer of CD8+ T cells from OTI/6PGD−/− and control OTI/6PGDf/f mice (Figure 3D). Recipients of OTI/6PGD−/− CD8+ T cells showed significantly smaller tumors...