Application Note: Isolation of circulating tumor cells using Dynabeads magnetic beads Flyer: Automated sample preparation for DNA, RNA, proteins, and cells KingFisher Apex script for automated enrichment of CTCs by leukocyte depletion (negative isolation) ...
安全数据表 SDS Scientific Resources Application Note: Isolation of circulating tumor cells using Dynabeads magnetic beads Brochure: Cell Isolation with Dynabeads Magnetic Beads Product Information User Guide: Dynabeads MyOne CD45 Leukocyte Depletion
Resuspend the beads by vortexing, maximum speed, 5 touches. Add 10µL of Nanobeads, mix well and incubate on ice for 15 minutes. Scale up the volume accordingly if separating more cells. For example, add 100µL for 1 x 108 cells. When working with less than 107 cells, use indicated...
Depletion of naive T cells using clinical grade magnetic CD45RA beads: a new approach for GVHD prophylaxis. Bone Marrow Transplant 2014;49:138-44.Teschner D, Distler E, Wehler D, Frey M, Marandiuc D, Langeveld K, et al. Depletion of naive T cells using clinical grade magnetic CD45RA ...
Depletion of CD45+ cells was done weekly for 3 weeks using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. Stromal cells were allowed to reach 80%–90% confluency and were subcultured for no more than 8 passages. Bone marrow-derived cells were ...
Purified T cells were prepared by using immuno-magnetic negative selection to deplete B cells and I-A+APC, as described previously[12]. Briefly, lymphocytes were allowed to react with an anti- I-Ad/b monoclonal antibody (mAb) and then incubated with magnetic beads bound to goat anti-mouse ...
Mouse lymph node B cells were purified by negative selection with CD11b- and CD43-conjugated magnetic beads (Miltenyi Biotec). Purified B cells were resuspended in serum-free RPMI (108 per ml) and activated by 5 μg/ml goat anti-mouse IgM [F(ab′)2] (Jackson Immunoresearch). Aliquots of...
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whole blood or peripheral blood mononuclear cells, utilizing a biotinylated Anti-Human CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by ...