For example, when using the Dynabeads magnetic beads M-450 CD4 positive isolation or depletion kit, a 4:1 bead-to-target cell ratio should be maintained. To capture 95% of target cells for molecular applications, the bead concentration must always be 1 x 10e7 beads per milliliter of ...
Resuspend the beads by vortexing, maximum speed, 5 touches. Add 10µL of Nanobeads, mix well and incubate on ice for 15 minutes. Scale up the volume accordingly if separating more cells. For example, add 100µL for 1 x 108 cells. When working with less than 107 cells, use indicated...
The Dynabeads MyOne CD45 Leukocyte Depletion magnetic beads process approximately 2.5 x 108 PBMCs per mL of beads. Features and benefits Efficient depletion of human leukocytes from PBMC and whole blood Enrichment of viable and untouched CTCs by leukocyte depletion High depletion efficiency of contamin...
英文名称: CD45 magbeads 保质期: 一年 个月 保存条件: 2-8℃ ★ BioMag -Anti CD45抗体包被磁珠 一、概述: 抗CD45抗体免疫磁珠由高性能的特异性 IgG1 单克隆抗体与磁珠共价偶联,后期经过封闭处理,可以直接用于捕获人的CD45细胞,具有较高的CD45细胞结合量以及非特异结合低的特点,可用于人的CD45细胞的...
3076596Certificate of Analysis2025年1月26日11170D 显示5 条结果, 在上面搜索特定证书 申请COA 安全数据表 SDS Scientific Resources Brochure: Cell Isolation with Dynabeads Magnetic Beads Product Information User Guide: Dynabeads MyOne CD45 Leukocyte Depletion...
The MagniSort™ Human CD45 Depletion Kit is designed to deplete CD45+ cells from human lysed whole blood or peripheral blood mononuclear cells, utilizing a biotinylated Anti-Human CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. ...
使用BDCompBeads进行仪器性能验证时,FITC通道的CV值应小于2%,PE通道检测灵敏度需达到<100MESF。当处理冻存样本时,CD45表达可能下降15-20%,建议增加抗体用量至1.5μg/10^6细胞。在胞内染色方案中,破膜剂会导致荧光强度衰减30%,需相应延长染色时间至45分钟。 答案与解析: 1.FITC标记CD45的激发波长:488nm(20%)...
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Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC Human TruStain FcX™ (Fc Receptor Blocking Solution) FC,ICC,ICFC Lymphopure™ Cell Sep - Pos,Cell Sep - Neg Compensation Beads FCRelated Pages & Pathways Pathways B cell Development and Activation B-Cell Receptor Pathway...
Compensation BeadsFC Tandem StabilizerFC Related Pages & Pathways Pathways B cell Development and Activation B-Cell Receptor Pathway CD28 Signaling in T-Helper Cell ICos-ICosL Pathway in T-Helper Cell Innate Immunity Regulatory T Cells T-Cell Receptor Signaling ...