Depletion of CD45+ cells was done weekly for 3 weeks using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. Stromal cells were allowed to reach 80%–90% confluency and were subcultured for no more than 8 passages. Bone marrow-derived cells were ...
Mouse lymph node B cells were purified by negative selection with CD11b- and CD43-conjugated magnetic beads (Miltenyi Biotec). Purified B cells were resuspended in serum-free RPMI (108 per ml) and activated by 5 μg/ml goat anti-mouse IgM [F(ab′)2] (Jackson Immunoresearch). Aliquots of...
免疫磁珠分选系统(Miltenyi Biotec),激光共 聚焦显微镜(Nikon EZ-C1),PCR 仪(Roche Light Cycler??R480)。 1.2 小鼠骨髓 CD45-细胞纯化、扩增 随机取 5 只小鼠经 2.5%戊巴比妥钠腹腔注射麻醉后,70%乙 醇浸泡 30 min,超净台无菌条件下取出小鼠股骨和胫骨数根, 用低糖 DMEM 反复冲洗髓腔,收集冲洗液,经 400...
miltenyi 美天旎 磁珠及其他分选产品130-056-701CD34多排序试剂盒 上海臻诺生物科技有限公司 3年 查看详情 ￥8798.00/件 上海 130-095-130美天旎磁珠分选产品小鼠Pan T细胞分选试剂盒II 细胞 美天旎品牌 上海希言生物科技有限公司 1年 查看详情 ￥680.00/盒 上海 海狸Beaver磁珠 质粒DNA小量抽提试剂盒 MN-P...
miltenyi 美天旎 磁珠及其他分选产品 130-056-701CD34多排序试剂盒 上海臻诺生物科技有限公司 3年 查看详情 ￥0.56/pcs 广东东莞 晶体管 电阻器 电感器 0805L035YR 晶振 磁珠 接线座 卡槽 三极管 连接器 官方品牌 北京京北通宇电子元件有限公司 5年 查看详情 ￥100.00/瓶 北京 碧云天 BeyoMag™...
To examine the activation status of T cells and monocytes, CD4+ T cells were depleted from CNS- isolated mononuclear cell pools using CD4 Dyn- abeads according to the manufacturer's instructions (Dynal Biotech, Oslo, Norway). CD4+ T cells were washed with flow buffer, filtered, and examine...
International Journal of Molecular Sciences Article Robust Identification of Suitable T-Cell Subsets for Personalized CMV-Specific T-Cell Immunotherapy Using CD45RA and CD62L Microbeads Caroline Mangare 1, Sabine Tischer-Zimmermann 1,2, Sebastian B. Riese 1, Anna C. Dragon 1 , Immo Prinz 3, ...