For example, when using the Dynabeads magnetic beads M-450 CD4 positive isolation or depletion kit, a 4:1 bead-to-target cell ratio should be maintained. To capture 95% of target cells for molecular applications, the bead concentration must always be 1 x 10e7 beads per milliliter of ...
The Dynabeads MyOne CD45 Leukocyte Depletion magnetic beads process approximately 2.5 x 108 PBMCs per mL of beads. Features and benefits Efficient depletion of human leukocytes from PBMC and whole blood Enrichment of viable and untouched CTCs by leukocyte depletion High depletion efficiency of contamin...
英文名称: CD45 magbeads 保质期: 一年 个月 保存条件: 2-8℃ ★ BioMag -Anti CD45抗体包被磁珠 一、概述: 抗CD45抗体免疫磁珠由高性能的特异性 IgG1 单克隆抗体与磁珠共价偶联,后期经过封闭处理,可以直接用于捕获人的CD45细胞,具有较高的CD45细胞结合量以及非特异结合低的特点,可用于人的CD45细胞的...
10 µl Mouse CD45 Nanobeads in 1x107 cells in 100 µl of buffer. Application Notes This kit is designed for the positive selection or depletion of CD45+ cells from lymphoid or different organ tissues. For depletion of CD45+ cells a higher volume of beads (up to 15 µl) and ...
Count and adjust the cell concentration to 1 x 108 cells/mL. Aliquot 100 µL of cell suspension (107 cells) into a new tube. Resuspend the beads by vortexing, maximum speed, 5 touches. Add 10µL of Nanobeads, mix well and incubate on ice for 15 minutes. Scale up the volume ...
MagniSort™ Streptavidin Negative Selection Beads 200 tests 8804-6810-74 MagniSort™ Human T cell Enrichment Kit 200 tests 8802-6831-74 MagniSort™ 人 CD4 T 细胞 2-Step富集试剂盒 200 tests 8804-6867-74 MagniSort™ Human B Cell Enrichment Kit (2nd Generation) 200 tests...
使用BDCompBeads进行仪器性能验证时,FITC通道的CV值应小于2%,PE通道检测灵敏度需达到<100MESF。当处理冻存样本时,CD45表达可能下降15-20%,建议增加抗体用量至1.5μg/10^6细胞。在胞内染色方案中,破膜剂会导致荧光强度衰减30%,需相应延长染色时间至45分钟。 答案与解析: 1.FITC标记CD45的激发波长:488nm(20%)...
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Beads | abt beads technologies | abvision | abx | abzoom | academy biomed | academy bio-medical | acanthus research | acanthusresearch | accelagen | Access Biologicals, LLC | accessbiologicals | acciusa | accu-chek | accumax accutase | Accurate | accurate chemical | Accurate Wirecraft Company...
Depletion of CD45+ cells was done weekly for 3 weeks using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. Stromal cells were allowed to reach 80%–90% confluency and were subcultured for no more than 8 passages. Bone marrow-derived cells were ...