The effective concentrations for platelet activation are in agreement for the two assay types, proving that platelet responsiveness can be quantified using antibody-coated magnetic beads. We discuss the outlook for application in lab-on-a-chip devices....
(AuNPs). AuNPs is characterized with a bright red color when free floating in solution. Accumulation ofantibody conjugatedAuNPs on a spot or line coated with antigen will result in visible color. Hence this technique is extensively applied inlateral flow assayto allow rapid, point-of-care ...
The streptavidin coated beads (5.18 μm, SiO2-MAG-SA-S1964, Microparticles) were used for the display of the SNAP-scFv-HA construct. Each selection round was performed according to the procedure described by Diamanteet al.31, with the following modifications: 1) improvement of the emulsion...
6 nmol of antigen was immobilized on streptavidin-coated magnetic beads (Thermo Fisher Scientific, USA). The beads were blocked by TBS/4%BSA. Phage library was mixed with the antigen-coated beads in 800 μL TBS/4%BSA. The supernatant was washed out by TBS /0.1% Tween20 twice and TBS o...
Purification from mouse serum is performed using streptavidincoated magnetic beads. Antigen-specific capture, based on recombinant biotinylated HER2, was successful in retaining trastuzumab emtansine on the beads. T-DM1 was eluted under acidic conditions in an aqueous environment and separated by reversed...
along with the potential for (non-covalently) adsorbed ligand to “leak” off the beads. To help prevent any “leakage” of non-coupled (non-covalently bound) ligand in the downstream assay, a couple of additional bead washing steps can be performed at the end ...
laser capture microdissection and fluorescence-activated cell sorting (FACS) can be applied. Alternatively, selection of antigen-specific B cells can be performed using antigen coated magnetic beads, fluorochrome-labeled antigens via multi-parameter FACS, the hemolytic plaque assay and a fluorescent foci...
[51]. In brief, plates were coated with Pluripro (Cell Guidance Systems) for at least 3 h. The cortex and hippocampus of each mouse were collected and washed in modified Hank’s Balanced Salt Solution. Tissue was then incubated with 0.5% trypsin for 20 min at 37 °C, followed by ...
In this assay, peptides that bind to the antibody block activity against a coated antigen (gp120 or gp41); peptides with mutations in key epitope residues will no longer bind the antibody resulting in an increased IC50 value as compared with a wild-type peptide, and peptides that have ...
Biotin-labelled lysate was coupled to 20 µl streptavidin coated magnetic beads (Roche Diagnostics) on a rotating wheel for 30 minutes at RT. Unbound lysate was removed using a magnetic concentrator and the beads washed twice with lysis buffer and once with M-PBS. Tomlinson I+J libraries...