Through the procedure, VANGL1 level from cell lysates of Caco2 and HT29 cells were quantified to estimate whether the antibody-conjugated magnetic bead can be used to trace amounts of VANGL1. Results from VANGL1
For example, the CellSearch System™ (Veridex), the only FDA-approved technique for CTCs detection, utilizes anti-EpCAM coated magnetic beads for CTCs capturing and enrichment, followed by using immunostaining of cytokeratins (CKs) for CTCs identification [1], [2], [3], [4], [5]. More...
IP protocol with magnetic beads Other "OTI2A11" antibodies (4) SKUDescriptionSizePrice CF810439 Carrier-free (BSA/glycerol-free) AFAP1 mouse monoclonal antibody,clone OTI2A11 100 ug $600.00 Add to Cart TA810439 AFAP1 mouse monoclonal antibody,clone OTI2A11...
The double-stranded DNA (dsDNA) PCR products were isolated from the reaction solution using streptavidin-coated sepharose beads (GE Healthcare, Chicago, IL, USA). After denaturation in 0.2 M NaOH, the Cy5-labeled ssDNA pools were desalted, lyophilized, and used for the next round of selection...
IP protocol with magnetic beads Other "OTI1F2" antibodies (1) SKUDescriptionSizePrice TA600381 purified TP63 mouse monoclonal capture antibody, validated for ELISA and Luminex assays 100 ug $458.00 Add to Cart Related Products Check items to add to...
In this study, we developed PSA antibody-conjugated microsphere beads that can be used for quantitation of PSA. We optimized the procedure for bead preparation and validated the detection analysis by using LNCaP and DU-145 prostate cancer cell lysates. By applying the procedure, extracellular PSA ...
iron nitride magnetic particlesT cell separationimmune magnetic beadsImmunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the ...
The immuno-magnetic beads conjugated with high-affinity mAb (clone 1456) appeared to have very low auto-fluorescence. Sufficient enrichment of Ep-CAMpositive cells using immuno-magnetic beads was observed in both simulation and clinical samples. The overall sensitivities of flow cytometry and imaging ...
Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 ...
The rate of retrieval ofendometrial cells using the immuno-magnetic beads was calculated. Endometrialcells were isolated using the immuno-magnetic beads from the vaginal dischargesof 22 patients with endometrial cancer and 16 non-malignant controls. Theisolated cells were stained using endometrial cancer...