CD4 (including monocytes) 50-55% 20 µL CD8a (including low expressing cells) 15-20% 10 µL CD19 5-10% 4 µL TCR Vδ9 1-3% 2 µLFor low frequency cells, pre-dilute the Nanobeads in order to pipette a minimum of 5 µL of any solution. For example, to isolate TCR V...
Mesenchymal cells were selected with anti-CD49a and/or anti-CD45 antibodies using either flow cytometry or a magnetic beads method. All CFU-F were always detected in the small population of CD49a-positive cells. These CFU retained their differentiation potential and gave rise to osteo-chondro...
Ms X Hu CD4 FITC 件 15天 会员价请登录 0 加入购物车 RH167059 CBL497 默克密理博/Millipore Ms X Hu CD49e clone NKI-SAM-1 件 15天 会员价请登录 0 加入购物车 RH167060 CBL517 默克密理博/Millipore Ms X Hu CD28, clone 15E8 件
antigens present on contaminating cells and not on CD4+ cells such asCD14,CD16,CD36, and CD123. Moreover, these antibodies are bound on dextran-coated magnetic beads. Subsequent exposure to a strongmagnetic fieldremoves the unwanted bead-coated cells, leaving behind the desired cell population....
Furthermore, 1G11 treatment improves CD3+, CD4 + and CD8 + T cell infiltration in tumor tissue. Conclusions We developed antagonist antibodies targeting LIF/LIFR signaling pathway from a naive human scFv phage library. Antagonist anti-LIF antibody exerts antitumor effects by specifically ...
Human PBMCs were isolated from the peripheral blood of healthy donors by standard density gradient centrifugation on Ficoll-Hypaque (GE Healthcare-Life Sciences, USA). Memory CD4+T cells were enriched by magnetic beads separation (Memory CD4+T Cell Isolation Kit; Miltenyi Biotec, USA) according ...
CD3-APC H7 (SK7, BD Pharmingen, San Diego, CA), CD4-APC (OKT4, eBioscience), CD8-PerCP Cy5.5 (SK1, BioLegend, San Diego CA), CD19-Brilliant Violet 605 (HIB19, BD Horizon, San Diego, CA), CCR5-PECy7 (2D7, BD Pharmingen), and Countbright beads (Invitrogen, Grand Island, NY...
Mix in proportion CD4+T cells and DC cells that are separated by magnetic beads and lay the mixture on the plate. Then add different concentrations of anti-PD1 mouse monoclonal antibody. After 5 days of culture, detect the concentration of IFN-γ by the kit. The results, as shown in FIG...
Significantly, the reactivity of the MAbs includes the ability to dose-dependently block CD27 interaction with CD70, reduce CD27 signaling in the presence of CD70, reduce IL-4 and IFNg production by T-cells, and inhibit CD70-dependent human naïve CD4+ T-cell proliferation, CD70-dependent ...
It was proved that pDC is a CD4+CD11c−2-type dendritic cell precursor cell, and that it produces IFN by 200 to 1,000 times greater than that produced by other blood cells after stimulation by a microorganism. Therefore, pDC2 is a decisive immune system effector cell in antiviral and ...