, 1989). However, the ability of CD8 to negatively regulate TCR-mediated signaling, possibly through sequestration of p56lck, was demonstrated in a system in which CD4−8− thymocytes from TCR transgenic mice were reconstituted with a transgenic CD8α molecule (van Oers et al., 1993). ...
B cells and TCR avidity determine distinct functions of CD4+ T cells in retroviral infection. J. Immunol. 187, 3321–3330 (2011). Article CAS PubMed Google Scholar Vanguri, V., Govern, C. C., Smith, R. & Huseby, E. S. Viral antigen density and confinement time regulate the ...
T cells were isolated from the lymph nodes and spleens of C7 or C24 TCR transgenic mice. Single cell suspensions were made by passing spleens and lymph nodes through 70 µm and 40 µm filters sequentially, and a MojoSortTM CD4 T cell isolation kit and magnet (Biolegend, San Dieg...
APCs at the CNS barriers and/or parenchyma are essential for locally executing and regulating T-cell responses via MHC–TCR interactions [22, 25]. Our RNA-seq studies demonstrate upregulated genes related to CD4 T-cell activation (e.g., CD44, CD81 and Il2rg) along with upregulated genes ...
Here we determined whether this combination of ionomycin and PMA induces differentiation of isolated DP thymocytes from normal and TCR transgenic mice. We found that pretreatment of DP thymocytes with ionomycin and PMA followed by 1 day culture of the cells without the reagents resulted in the ...
Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells Xuyen T. Dinh Dragana Stanley Margaret A. Jordan Scientific Reports(2021) Inducible de novo expression of neoantigens in tumor cells and mice ...
Teh, H. S. et al. Thymic major histocompatibility complex antigens and the αβ T-cell receptor determine the CD4/CD8 phenotype of T cells.Nature335, 229–233 (1988).The first use of TCR-transgenic mice to reveal the relationship between MHC class-specificity in foreign antigen recognition ...
If specific TCRs are associated with the appearance of CD4 CTLs during infection, then these TCRs could be cloned and used to generate TCR transgenic mice. This might open up new experimental strategies towards assessing CD4 CTL function in vivo. Moreover, considering Th subset plasticity [6,...
T-cell receptor transgenic (TCR-Tg) mice from two diabetogenic CD4+ islet-antigen-specific T-cell clones 来自 NCBI 喜欢 0 阅读量: 85 作者: K Haskins 摘要: Haskins K.DOI: 10.1016/j.jaut.2003.10.006 被引量: 29 年份: 2004 收藏 引用 批量引用 报错 分享 全部来源 免费下载 求助全文 全文...
Virus-specific MHC-class II-restricted TCR-transgenic mice: effects on humoral and cellular immune responses after viral infection. Eur. J. Immunol. 28, 390–400 (1998). Article CAS Google Scholar Oxenius, A. et al. Presentation of endogenous viral proteins in association with major ...