(Product # 11-0149-42-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 661 (Product # 376-4714-81) (left) or CD33 Monoclonal Antibody, Brilliant Ultra Violet 661 (right). Viable cells in the monocyte gate were used for analysis, as determined by 7-AAD (Product # 00-...
MonocyteCD33ApoptosisInhibitory receptorAlthough CD33 represents an important marker of myeloid cell differentiation, its function remains poorly defined. In view of its homology with p75/AIRM1, a recently identified surface molecule which exerts a potent inhibition on NK cell function, we re-evaluated ...
Expression of CD33-related siglecs on human mononuclear phagocytes, monocyte-derived dendritic cells and plasmacytoid dendritic cells 2004, Immunobiology Show abstract Basic and clinical immunology of Siglecs 2008, Annals of the New York Academy of Sciences Siglecs and their roles in the immune system ...
Decreased protein levels of hCD33M was observed by Western blot and flow cytometry on peripheral blood monocytes and monocyte-derived microglia like cells [7, 44] as well as leukemic blasts from acute myeloid leukemia (AML) patients [42] homozygous for the AD-protective CD33 SNP (rs3865444A/...
2B). Gates were defined based on established side- and forward-scatter positions for myeloid and monocytic cells [5], and based on bright CD16 staining (metamyelocyte/neutrophil marker) preferentially in gate A and bright CD14 staining (monocyte marker) preferentially in gate B (data not shown...
Dose dependent monocyte depletion in PBMC by tested mono- and bispecific TCE is shown as CD14% determined in flow cytometry (B, 1 representative donor out of 8 is shown). The global geometric EC50 (half-effective dose) derived from the dose response curves of 8 different donors is shown to...
Manipulation of sequences and alignments were performed by using Baylor College of Medicine molecular biology software available on the internet (Human Genome Center, Baylor College of Medicine, Houston, TX). Northern blot analysis. The cDNA insert from pHMQCD14 was labeled with 32P by using the ...
(Product # 11-0149-42-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 661 (Product # 376-4714-81) (left) or CD33 Monoclonal Antibody, Brilliant Ultra Violet 661 (right). Viable cells in the monocyte gate were used for analysis, as determined by 7-AAD (Product # 00-...
Two problems remain: First, the incomplete removal of nonlymphoid cells when CD14 is used to exclude them from the lymphocyte gate. Second, the lack of a restricted marker to identify NK cells that are usually defined as CD3-/CD16/56+ lymphocytes, though contaminating monocyte subsets share ...
May-Grünwald-Giemsa cytospin results showed that the morphological features of HLA-DR−/lowCD33+CD16− cells were similar to those of immature monocyte-like cells (Fig. 1e). The in vitro immune-suppressive activity of the HLA-DR−/lowCD33+CD16− population identified among the G-PBSC...