Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, FITC (Product # 11-0149-42-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 661 (Product # 376-4714-81) (left) or CD33 Monoclonal Antibody, Brilliant Ultra Violet 661 (right). Viable cells in th...
Monocyte differentiation in intestine-like macrophage phenotype induced by epithelial cells by immunohistochemistry and flow cytometry, were down-regulated after 7 days (e.g., for CD14 at 24 h, expression was 86% of CD33+ cells; at day ... T Spöttl,M Hausmann,M Kreutz,... - 《Journal...
Expression of CD33-related siglecs on human mononuclear phagocytes, monocyte-derived dendritic cells and plasmacytoid dendritic cells 2004, Immunobiology Show abstract Basic and clinical immunology of Siglecs 2008, Annals of the New York Academy of Sciences Siglecs and their roles in the immune system ...
Thus, in addition to inducing pLAIR-1, a direct biological effect of C1q on monocyte function may be to activate CD33 inhibitory signaling. Figure 3 C1q triggers CD33 ITIM phosphorylation and CD33-LAIR-1 physical associations in human monocytes. Monocytes were either untreated (utx), treated ...
Two problems remain: First, the incomplete removal of nonlymphoid cells when CD14 is used to exclude them from the lymphocyte gate. Second, the lack of a restricted marker to identify NK cells that are usually defined as CD3-/CD16/56+ lymphocytes, though contaminating monocyte subsets share ...
May-Grünwald-Giemsa cytospin results showed that the morphological features of HLA-DR−/lowCD33+CD16−cells were similar to those of immature monocyte-like cells (Fig.1e). The in vitro immune-suppressive activity of the HLA-DR−/lowCD33+CD16−population identified among the G-PBSC was...
(Product # 11-0149-42-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 661 (Product # 376-4714-81) (left) or CD33 Monoclonal Antibody, Brilliant Ultra Violet 661 (right). Viable cells in the monocyte gate were used for analysis, as determined by 7-AAD (Product # 00-...
To investigate the interaction between CD33 and SHP-1, proximity ligation assay (PLA) was performed on primary monocyte-derived microglia-like (MDMi) cell cultures from individuals genotyped for the CD33 AD-associated variant, rs3865444. As PLA detects protein–protein interactions in situ at a di...