Recently, powerful biological tools (CCR2-red fluorescent protein [RFP] knock-in mice) have been developed to analyze the functions of CCR2 in different cell populations, and intriguing results have emerged from those mice. The present review emphasizes CCR2/CCL2 as a key chemokine/chemokine ...
[32]. Recent evidence suggests that the highly phagocytic and inflammatory macrophages produced by blood-derived CCR2rfp/+monocytes elicitdemyelinationin EAE. According to Nielsen et.al., assessment of the demyelinating effect of CCR2+monocytes on cortical shows: Th/+CCR2−/−mice developed EAE...
Cell suspensions of the peripheral blood, BM, spleen and cervical LNs from the CCR2 RFP reporter mice (CCR2-RFP+/−) and age-and sex-matched non-transgenic WT C57BL/6J mice were stained with differential subset-specific mAbs. In flow cytometric analyses, subset identification was inflammatory...
Ccr2 RFP/RFP mice display reduced infiltration of Ly6Chi inflammatory monocytes into the brain after TBI [25]. Here, Cx3cr1 GFP/GFP mice showed no difference in TBI pathology compared to wild type (WT) mice, but Ccr2 RFP/RFP mice had reduced lesion volume and axonal pathology. ...
CCR2RFP macrophages were identifi- able in the cortex, cerebellum, and the hippocampus of ethanol-fed mice (Fig. 2b). Although there was a general trend toward an increase in the number of peripheral CCR2RFP+ IMs after chronic alcohol in all three brain re- gions, IMs were twice as ...
Whereas NK cells in all tissues did not differ between two strains, NK T cells in the blood and spleen, and T cells in the blood and spleen were significantly more prevalent in CCR2-RFP+/+ mice than in CCR2-RFP+/− mice. B cells were also more prevalent in BM but lower in the...
Adult 3- (young) and 23- month-old (aged) male and female CX3CR1GFP/+CCR2RFP/+ (double heterozygous (Dbl-Het)) and C57BL6/J (wild type (WT)) male mice were used for all experiments. Dbl-Het mice (n = 16) were generated as previously described [12] and genotyped using a ...
To test this hypothesis, a mouse with red fluorescent protein (RFP) driven by the chemokine receptor 2 (CCR2), was treated with HFHF or control diet (CD) for five weeks to induce chronic inflammation. CCR2 is expressed on peripheral monocytes and to a lesser degree on microglia in the ...
To test the specificity of FACS staining for CCR2, we examined HSCs in CCR2+/RFP mice, in which we detected a similar RFP+ HSC frequency of 15% (Figure S1E). CCR2+CD150+CD48− LSKs also contain higher levels of CCR2 mRNA when compared to their CCR2− counterparts (Figure S1E)...
DRGs of mice infected with either NU14 or SΦ874/pDgal expressed only low levels of MCP-1-RFP but high levels of CCR2-GFP in DRG cell bodies. These results suggest that both MCP-1 and CCR2 are upregulated in response toE. colithat induce post-UTI chronic pain, but bladder infection...