Cell suspensions of the peripheral blood, BM, spleen and cervical LNs from the CCR2 RFP reporter mice (CCR2-RFP+/−) and age-and sex-matched non-transgenic WT C57BL/6J mice were stained with differential subset-specific mAbs. In flow cytometric analyses, subset identification was inflammatory...
However, subsets of T cells, NK cells, and Ly6Clo monocytes express CCR2 or the RFP reporter in some conditions [26]. Although the frequency of these other CCR2/RFP+ cells is significantly lower than the frequency of CCR2+ inflammatory monocytes after TBI [24], we cannot rule out that ...
c Using Dbl-Het reporter mice (n = 6/group), macrophages (CD11b+F4/80+) were delineated based upon their relative expression of GFP (CX3CR1) from RFP (CCR2) by flow cytometry. There were relatively very few CCR2+ macrophages (blue box) in the sham animals; however, there was a ...
DMM surgery was performed in the right knee of 10-week-old male wild-type (WT), Ccr2 null, or Ccr2RFP C57BL/6 mice. Knee hyperalgesia was measured using a Pressure Application Measurement device. CCR2 receptor antagonist (CCR2RA) was injected systemically (i.p.) or intra-articularly (...
18. DRGs were isolated from reporter mice at times corresponding to allodynia (i.e., PID 2 for saline controls and NU14-infected mice, PID 14 for SΦ874- and SΦ874/pDgal-infected mice) and then examined for MCP-1-RFP and CCR2-GFP expression by confocal fluorescence microscopy in ...
CCR2-expressing cells were defined as RFP-positive cells. In each graph, number represents the percentage of RFP-expressing CCR2-positive cells in a given subset. Solid and dashed lines indicate the CCR2 RFP protein expression in CCR2 RFP reporter mice and background autofluorescence staining in...