However, distinguishing between microglia and macrophages is difficult using conventional techniques such as cell markers (e.g., Iba-1). Recently, two mouse models for visualizing chemokine receptors were established, Cx3cr1 (GFP/GFP) and Ccr2 (RFP/RFP) mice. As Cx3cr1 is expressed in ...
Ccr2 RFP/RFP mice display reduced infiltration of Ly6Chi inflammatory monocytes into the brain after TBI [25]. Here, Cx3cr1 GFP/GFP mice showed no difference in TBI pathology compared to wild type (WT) mice, but Ccr2 RFP/RFP mice had reduced lesion volume and axonal pathology. ...
Adult 3- (young) and 23- month-old (aged) male and female CX3CR1GFP/+CCR2RFP/+ (double heterozygous (Dbl-Het)) and C57BL6/J (wild type (WT)) male mice were used for all experiments. Dbl-Het mice (n = 16) were generated as previously described [12] and genotyped using a ...
Ccr2-RFP single positive bone marrow-derived macrophages were observed to migrate into the retina of Mertk (-/-) Cx3cr1 (GFP/+) Ccr2 (RFP/+) mice. These invading cells were still observed in the subretinal space in 18-week-old animals.Cx3cr1-GFP-positive microglia and Ccr2-RFP-...
Methods :Mertk-/-Cx3cr1GFP/-Ccr2RFP/- mice received once-daily intraperitoneal injections of minocycline at a dose of 50 mg/kg (Mino50), 100 mg/kg (Mino100), or PBS as control, starting from postnatal day 28 (P28) and continuing through to P42. Slide-mounted sections of eyes at ...
Bone marrow from Ccr2RFP/WT/Cx3cr1GFP/WT mice was cultured in the presence of KR158B glioma conditioned media followed by fluorescent microscopy and flow cytometry analysis to assess M-MDSCs. T cell suppression assay was conducted using Ly6G-/GR1+ cells (M-MDSCs) isolated from bone marrow...
METHODS. Immune cell characteristics were determined in CCR2 antagonist (CCX872)/anti-PD-1 treated KR158 or 005 GSC glioma-bearing wild type or CCR2+/rfp/CX3CR1+/gfp reporter mice. RESULTS. CCX872 increased median survival (32 vs. 50 days, P=.002) in KR158 tumor-bearing animals and...
METHODS. To identify the source of CCR2+/CX3CR1+ myeloid cells, chimeric wild type mice harboring bone marrow cells from transgenic CCR2WT/RFP/CX3CR1WT/GFP mice were generated. CCR2+/CX3CR1+ cells were enriched from bone marrow obtained from either wild-type or CCR2WT/RFP/CX3CR1WT...
Therefore, we took advantage of Cx3cr1GFP and Ccr2RFP reporter mice, in which the respective gene was replaced by a fluorescent reporter protein gene. We analyzed the expression of CX3CR1 and CCR2 by flow cytometry using several validated fluorochrome-coupled antibodies and compared them with...