Generation and characterization of CCR2RFP mice.Noah, SaederupAstrid, E. CardonaKelsey, CroftMakiko, MizutaniAnne, C. CotleurChiaLin, TsouRichard, M. RansohoffIsrael, F. Charo
Ccr2RFP mice and immunohistochemical staining for the pan-neuronal marker, protein gene product 9.5 (PGP9.5), or the sensory neuron marker, calcitonin gene-related peptide (CGRP), were used to visualize the location of CCR2 on intra-articular afferents. Results WT, but not Ccr2 null, mice ...
To test the specificity of FACS staining for CCR2, we examined HSCs in CCR2+/RFP mice, in which we detected a similar RFP+ HSC frequency of 15% (Figure S1E). CCR2+CD150+CD48− LSKs also contain higher levels of CCR2 mRNA when compared to their CCR2− counterparts (Figure S1E)...
Although there was a general trend toward an increase in the number of peripheral CCR2RFP+ IMs after chronic alcohol in all three brain re- gions, IMs were twice as numerous in the hippocampus in the ethanol-fed mice (Fig. 2c). Interestingly, there was no change in the number of CX3...
Brain sections from CCR2-RFP mice were stained by immunofluorescence in the hippocampal areas of CA1, CA3, and the dentate gyrus as these areas are affected in the neurodegenerative Alzheimer's Disease. CD169 and CCR2 stained cells were quantified using mean intensity to determine the effects ...
In this study, we used red fluorescent protein (RFP) CCR2 reporter mice28 to evaluate the frequency and intensity of CCR2 expression on individual leukocyte subsets. Further, we determined the degree to which genetic deletion, or pharmacological inhibition of CCR2 with PG, alters the relative an...
[32]. Recent evidence suggests that the highly phagocytic and inflammatory macrophages produced by blood-derived CCR2rfp/+monocytes elicitdemyelinationin EAE. According to Nielsen et.al., assessment of the demyelinating effect of CCR2+monocytes on cortical shows: Th/+CCR2−/−mice developed EAE...
Monocyte subpopulations distinguished by differential expression of chemokine receptors CCR2 and CX3CR1 are difficult to track in vivo, partly due to lack of CCR2 reagents.We created CCR2-red fluorescent protein (RFP) knock-in mice and crossed them with CX3CR1-GFP mice to investigate monocyte...
c Using Dbl-Het reporter mice (n = 6/group), macrophages (CD11b+F4/80+) were delineated based upon their relative expression of GFP (CX3CR1) from RFP (CCR2) by flow cytometry. There were relatively very few CCR2+ macrophages (blue box) in the sham animals; however, there was a ...
Ccr2 RFP/RFP mice display reduced infiltration of Ly6Chi inflammatory monocytes into the brain after TBI [25]. Here, Cx3cr1 GFP/GFP mice showed no difference in TBI pathology compared to wild type (WT) mice, but Ccr2 RFP/RFP mice had reduced lesion volume and axonal pathology. ...