Here, we identify the splicing factor SRSF6 as a key factor in the AS response to hypoxia. The SRSF6 level is strongly reduced in acute hypoxia, which serves a dual purpose: it allows for exon skipping and triggers the dispersal of nuclear speckles. Our data suggest that cells use ...
Exon Skipping Exon Skipping, 或者叫做cassette exon, 称为外显子跳跃,示意图如下 ? 2. 3.1K20 文献阅读-MsbA的变构抑制机制研究 简介: ABC转运蛋白(ATP-binding cassette transporter)是一类膜蛋白质,它们能够利用ATP的能量将物质从细胞内或细胞外运输到对应的另一侧。
Thus, we were able to exclude exon 1 as a target as it encodes the MHC signal peptide that is cleaved upon expression, and exon 8, as it shares 100% nt. identity between the two alleles. Each gRNA was cloned into the CRISPR-Cas9 plasmid pX458 (pSpCas9(BB)-2A-GFP), where Cas9 ...
(Perhaps cut the leader a bit short so that the splicing tape never touches the heads.) When finished with these operations, ... S Collins 被引量: 4发表: 1985年 Alternative splicing of a short cassette exon in alpha1B generates functionally distinct N-type calcium channels in central and ...
Analysis of Cis-Regulatory Motifs in Cassette Exons by Incorporating Exon Skipping Rates Identification of cis-regulatory motifs has long been a hotspot in the study of alternative splicing. We propose a two-step approach: we first identify k-m... S Zhao,J Kim,S Heber - International Symposium...
The EF1αEP cassette has exon 1 and most of intron 1 of the EF1α enhancer/promoter which includes the transcription initiation site, while the Pless-nlslacZ cassette has a 0.2-kb remnant of the EF1α enhancer/promoter, the nlslacZ gene and the poly A signal. Two kinds of the EF1αEPs...
The HaloTag-TEV cassette was inserted in the 3ʹ end of titin’s exon 225, which codes for the I86 domain (residues 14072–14157, UniProt A2ASS6-1). I86 is located at the end of the I-band of titin (Fig. 1 and Supplementary Fig. 1). The HaloTag-TEV cassette includes a copy...
Using Affymetrix mouse exon-arrays, we identified exons with circadian alternative splicing in the liver. Validated circadian exons were regulated in a tis... NJ Mcglincy,A Valomon,JE Chesham,... - 《Genome Biology》 被引量: 88发表: 2012年 A new rhythm library for testing automatic external...
The exon–intron organization of each gene and locations of SNPs identified within each locus are illustrated schemati- cally in Fig. 1; detailed information is given in Table 1. The classification of SNPs on the basis of their locations is also summarized in Table 2. Among the 605 SNPs, ...
to prevent splicing of the genomic transcript and consisted of a single copy of the HS-40 enhancer, the β-globin gene promoter truncated to position −127, and the genomic elements of the Aγ-globin gene starting with the first exon and containing the large internal deletion of intron 2....