Here, we identify the splicing factor SRSF6 as a key factor in the AS response to hypoxia. The SRSF6 level is strongly reduced in acute hypoxia, which serves a dual purpose: it allows for exon skipping and triggers the dispersal of nuclear speckles. Our data suggest that cells use ...
V6 exon containing isoforms has been known to be implicated in tumor cell invasion and metastasis. In the present study, we performed SR protein screen for CD44 V6 splicing using overexpression and lentivirus-mediated shRNA treatment. Using......
One splice variant lacking the exon 28 corresponded to about 25% of total ABCC11 gene transcripts. Furthermore, four splicing variants encoding putatively short peptides were predominant in ABCC12 gene transcripts. Those splicing variants may represent diverse biological functions of these ABC ...
Identification of cis-regulatory motifs has long been a hotspot in the study of alternative splicing. We propose a two-step approach: we first identify k-mer seed motifs by testing for enrichment and significant differences in exon skipping rate, then a local stochastic search is applied to ...
1.9K21 可变剪切的意义和重要性 Exon Skipping Exon Skipping, 或者叫做cassette exon, 称为外显子跳跃,示意图如下 ? 2. 3.1K20 文献阅读-MsbA的变构抑制机制研究 简介: ABC转运蛋白(ATP-binding cassette transporter)是一类膜蛋白质,它们能够利用ATP的能量将物质从细胞内或细胞外运输到对应的另一侧。
Thus, we were able to exclude exon 1 as a target as it encodes the MHC signal peptide that is cleaved upon expression, and exon 8, as it shares 100% nt. identity between the two alleles. Each gRNA was cloned into the CRISPR-Cas9 plasmid pX458 (pSpCas9(BB)-2A-GFP), where Cas9 ...
The mouse Slc4a7 gene contains, and the human SLC4A7 gene is predicted to contain, a novel exon that encodes an alternatively spliced cassette IV of 20 aa in the cytoplasmic Nt domain of NBCn1. This new cassette IV is in a position homologous to that of a previously described cassette ...
Thus, regulation of the activities of the spliceosomal helicases may have a major impact on gene expression and regulation, in particular in higher eukaryotes, where alternative splicing is pervasive (26). A number of specific regulatory principles have been delineated for BRR2 (27). For example...
6.3 Causal near-exon variants in ABCA4Seven variants located near exons were found to result in splicing defects that affect neighboring exons (Table 4). Variants c.1937+13T>G, c.1937+37C>G, c.3191-11T>A and c.4352+61G>A create or strengthen intronic splice sites. They thereby ...
The EF1αEP cassette has exon 1 and most of intron 1 of the EF1α enhancer/promoter which includes the transcription initiation site, while the Pless-nlslacZ cassette has a 0.2-kb remnant of the EF1α enhancer/promoter, the nlslacZ gene and the poly A signal. Two kinds of the EF1αEPs...