SH-SY5Y and SK-N-BE(2) cells were transduced with SmartVector lentivirus (V3IHSHEG_6494503) containing a doxycycline-inducible shRNA cassette for TDP-43. Transduced cells were selected with puromycin (1 μg ml−1) for one week. For doxycycline dose–response experiments, the pool of TDP...
For example, the ψ value for a cassette exon is the fraction of all mRNAs that contain the cassette exon. The ψ value is independent of gene expression and falls within the range 0–100%. Serine/arginine-rich proteins (SR proteins). A family of splicing factors that frequently promote ...
GGC, GGU and GGA apparently rely on the first nucleotide in the pentamers to be A or U, to form AG-core or UG-type motifs, whereas CCU and CCA rely on the first nucleotide to be A or U to make GCWCC silencers. For Position 3-dependent MSEA, we analyzed all 3-mers at Position ...
CABLE SLEEVE BOX HAVING CASSETTE HOLDING DEVICE FOR HOLDING AND SPLICING LIGHT WAVE CONDUCTORPROBLEM TO BE SOLVED: To execute a splice system having ... S Michael,ミヒャエル シュターテツニー,FFL Froehlich,... 被引量: 0发表: 1998年 process and device for splicing optical cable to canduct...
SON is a nuclear speckle protein that is highly expressed in pluripotent cells and multiple cancer cell lines and promotes the inclusion of cassette exons with weak splice sites (Sharma et al., 2011). Interestingly, the reported DNA consensus sequence for SON binding (GA[GT]AN[CG][AG]CC) ...
A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast Genomic regions containing trinucleotide repeats (TNRs) are highly unstable, as the repeated sequences exhibit a high rate of mutational change, in...
Since exon PSI often changes across tissues (Pan et al., 2008), this allowed us to compare the effect on inclusion of the same genetic variants at different PSIs for 4,418 alternative cassette exons. sQTLs had smaller effects in tissues with low or high PSI and larger effects in tissues ...
We can see a direct correlation between expression of Fox2, a known splicing factor, and the alternative splicing of cassette exons containing binding sites for this protein in the neighbouring introns. Thus, our results suggest that the effects of alternative mRNA isoforms should now be ...
categories in mouse transcriptomes) and –log10 of fold enrichmentpvalues are provided for each AS category in the lower panel. 3SS, alternative 3′ splice sites; A5SS, alternative 5′ splice sites; ES, exon skipping; MIC, alternative micro cassette exon ≤15 nucleotides; IR, retained ...
The proportion of "cassette exon" is nearly double, while those of all the others decrease (See Figure 6). Other than the technical differences between the experiments, we hypothesize that the proportion of the splicing patterns may be specific to different phenotypic conditions. For example, the...