CRISPR-U™ 是源井生物自主研发的应用于基因编辑细胞系的独家技术(基于CRISPR / Cas9技术),通过优化基因编辑载体和基因编辑流程,CRISPR-U™ 技术的基因切割效率和重组效率是普通的CRISPR/Cas9技术的10倍以上,轻松实现细胞水平的基因敲除(KO)、基因点突变(PM)和基因敲入(KI)。利用CRISPR-U™ 的技术优势,源井生...
利用这一基因编辑技术可实现对基因组的精准编辑(敲除KO、敲入KI和点突变PM)。目前，KO细胞系的建立/KO...
通过CRISPR/Cas9技术制备特定基因修饰的小鼠模型。CRISPR/Cas9是目前最常用的基因编辑技术,可用于制备CKO/KO/KI/PM等类型基因编辑小鼠。 药康优势 1.高质量:执行最严格质控标准,首创Realtime PCR检测,0随机插入,0串联重组 2.高效率:GPTech优化高效的基因编辑平台,高一次成功率,交付周期更短 3.多修饰:靶基因多位点...
源井生物的CRISPR-U™技术，作为CRISPR/Cas9技术的升级版，显著提高了基因切割效率和重组效率，轻松实现基因编辑。通过CRISPR-U™技术，源井生物已成功在超过100种细胞系上实现了基因敲除（KO）、基因点突变（PM）和基因敲入（KI）。
(DNA High Sensitivity kit, sample dilution 1:5) to determine size distribution and a Qubit 3.0 fluorometer to determine concentration of libraries. Indexed DNA libraries were normalized to 4 nM, pooled and loaded at a concentration of 9 pM onto an Illumina Flowcell v2 with 19% of ϕX ...
W Qin,SL Dion,PM Kutny,... - 《Genetics》 被引量: 96发表: 2015年 Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is an adaptive immune system...
CRISPR-U™是源井生物自主研发的基因编辑技术(基于CRISPR / Cas9技术),CRISPR-U™技术比普通CRISPR/Cas9技术的基因切割效率更高,同时可以大幅度提升同源重组效率,轻松实现细胞和动物水平的基因敲除(KO)、基因点突变(PM)和基因敲入(KI)。利用CRISPR-U™的技术优势,源井生物已成功在超过70种细胞系上实现基因编辑...
van PM, van der Pijl EM, Hulsker M, et al. Low dystrophin levels in heart can delay heart failure in mdx mice. J Mol Cell Cardiol 2014; 69:17–23. Article Google Scholar Gulick J, Subramaniam A, Neumann J, Robbins J . Isolation and characterization of the mouse cardiac myosin heavy...
phaffii VKPM Y-5014 (Leu– phenotype) were derived from the K. phaffii VKPM Y-4287 strain, which has a high expression potential. Based on the developed recipient strains, markerless producers of heterologous proteins could be obtained. Efficiency of the gene inactivation with ...
PrimerDesign.pm - object used to design PCR primers for efficiency screening Primer.pm - object representing a PCR primer PrimerPair.pm - object representing a pair of PCR primers Cas9.pm - object representing a particular type of Cas9 ...