In our in vitro model, cancer cells expressing chicken ovalbumin (OVA) were co-cultured with OVA-specific CD8+T cells isolated from OT-I transgenic mice. The proliferation and function of CD8+ T cell were damped by tumor cells in an antigen dependent way. In our in vivo model, OVA-...
MDA-MB-231-LUC cells (2 × 106) were subcutaneously injected into the right inguinal mammary fat pad of the mice to establish a Breast cancer orthotopic transplantation model [55]. The bioluminescent signal of MDA-MB-231 cells was analyzed using the CRi Maestro in vivo imaging system (C...
As the11p15.4-11p15.5locus is subjected to parental genomic imprinting22, we analyzed the methylation status ofH19(a non-coding RNA tumor suppressor with maternal expression),IGF2-AS(insulin-like growth factor II gene, pro-oncogenic gene expressed only from the paternal allele) and theKCNQ1OT1...
but the phenotype could be masked under optimal culture conditions. Supporting this idea, we found thatOTUD5KO hESCs showed reduced growth in the E8 condition when the cells were passaged without the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Fig.4i), a compound normally...
In comparison with the normal wild‐type mice ( Fam83h W/W), the F2 homozygous KO ( Fam83h Ko/Ko) had sparse, scruffy coats with small body size and decreased general activity. Also, they had the natural reproductive ability and natural lifespan. In addition, delay in opening the eyes ...
(C) Frequency of indels by sgRNA-m3 at on-target (h-H530R) and off-target (h-OT1-h-OT10 and h-WT) sites in human WT and H530R ES cells. Data represent mean ± SD (n = 5). The most prevalent on-target indels of PRKAG2 H530R by sgRNA-m3 are shown in the lower panel....
Oberdoerffer, P., Otipoby, K. L., Maruyama, M. & Rajewsky, K. Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71. Nucleic Acids Res 31, e140 (2003). Article Google Scholar Fisher, E. M., Lana-Elola, E., Watson, S. D., Vassiliou, ...
the TS9 and many of the TS11 off-target sites. At two of the SpCas9 off-target sites (OT9-4 and OT9-8), the use of the attenuated SpCas9MT2-SaCas9WTnuclease dramatically reduces the off-target activity compared to the independent wild-type SpCas9 or the SpCas9WT–SaCas9WTnuclease,...
Cummings DM, Liu W, Portelius E, Bayram S, Yasvoina M, Ho S-H, Smits H, Ali SS, Steinberg R, Pegasiou C-M, James OT, Matarin M, Richardson JC, Zetterberg H, Blennow K, Hardy JA, Salih DA, Edwards FA (2015) First effects of rising amyloid-β in transgenic mouse brain: synap...
OT: Off-target PCD: Primary ciliary dyskinesia PFV: Prototype foamy virus qPCR: Quantitative PCR sgRNA: Single guide RNA SIN: Self-inactivating RRE: Rev-responsive element RT: Room temperature TRAC: T cell receptor alpha constant TU: Transduction unit VSV-G: Vesicular stomatitis ...