Rosa26- CAG-LSL-2xKRAB-dCas9 mice were crossed with Dppa3-Cre mice to delete STOP cassette. 图1 该小鼠含有loxp-STOP-loxp结构,是条件性过表达2XKRAB-dCas9,需要和Cre交配才能表达2XKRAB-dCas9 。该小鼠和广泛表达的Cre(Dppa3-Cre)交配后检测Cas9表达,westernblot检测发现Cas9在肝脏、脾、肺、肾、胸腺...
In addition, we demonstrated that myostatin gene in the thigh muscles of C57BL/10 mice was knocked out by the rAAV9-SaCas9 system to make muscle mass increased obviously. The protein eGFP into rAAV has significant implications for our indirect analysis of the editing efficiency of SaCas9 in...
et al. Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol 36, 888–893 (2018). Article CAS Google Scholar Jiang, W. et al. BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res 28, 855–861 (2018). ...
In addition, we demonstrated that myostatin gene in the thigh muscles of C57BL/10 mice was knocked out by the rAAV9-SaCas9 system to make muscle mass increased obviously. The protein eGFP into rAAV has significant implications for our indirect analysis of the editing efficiency of SaCas9 in...
*使用本品系发表的文献需注明: R26-CAG-LSL-dCas9-VPR-IRES-EGFP mice (Cat. NO. NM-KI-18004) were purchased from Shanghai Model Organisms Center, Inc.. 相关品系 Csf1r-Flox/R26-CreERT2 品系名:C57BL/6Smoc-Csf1rem1(flox)Gt(ROSA)26Sorem1(SA-FRT-2xpolyA-CAG-FRT-CreERT2-Rox-WPRE-Rox-...