Rosa26- CAG-LSL-2xKRAB-dCas9 mice were crossed with Dppa3-Cre mice to delete STOP cassette. 图1 该小鼠含有loxp-STOP-loxp结构,是条件性过表达2XKRAB-dCas9,需要和Cre交配才能表达2XKRAB-dCas9 。该小鼠和广泛表达的Cre(Dppa3-Cre)交配后检测Cas9表达,westernblot检测发现Cas9在肝脏、脾、肺、肾、胸腺...
Compared with AAV-DJ, AAV9 is more addicted in muscle tissue; it is conducive to gene editing of muscle cells and is often applied in muscle tissue. In addition, the packaging of different types of AAV allows for broad applicability of the psgMstn1 plasmid. 90 C57BL/10 male mice were d...
Remarkably, at E12.5, the graded expression patterns of Pax6 in the developing neocortex now become recognizable in our knock-in mice, serving a sufficiently sensitive and useful tool to precisely visualize neurodevelopmental processes.doi:10.1016/j.neures.2018.01.007Yukiko U. Inoue...
*使用本品系发表的文献需注明: R26-CAG-LSL-dCas9-VPR-IRES-EGFP mice (Cat. NO. NM-KI-18004) were purchased from Shanghai Model Organisms Center, Inc.. 相关品系 R26-CAG-LSL-tdTomato 品系名:C57BL/6Smoc-Gt(ROSA)26Sorem(CAG-LSL-tdTomato)1Smoc ...