Protein dysfunctions may be responsible for the most DNA damage caused by hydroxyl radicals, which can generate mutations, genetic instability, and epigenetic changes that can lead to cancer. In fact, the DNA damage marker 8-OHdG is mutagenic, and high levels of 8-OHdG have been found in vari...
This association results in constitutive normoxic degradation of the HIF-1α protein. Hypoxia, mutation of VHL, or accumulation of reactive oxygen species (ROS) or the TCA cycle intermediates succinate and fumarate impair HIF-1α degradation, allowing it to enter the nucleus and engage in ...
4e), which was fully corroborated by western-blot analysis of variation of TMED3 in MCF-7 and MDA-MB-231 cells on protein level (Fig. 4f, g). Additionally, expression level of miR-188-3p was detected using qRT-PCR in 37 paired cases of breast cancer and its matched normal controls. ...
Here, we uncover the mechanisms by which the transcription factor Carbohydrate responsive element binding protein (ChREBP) functions as an oncogene during hepatocellular carcinoma (HCC) development. Mechanistically, ChREBP triggers the expression of the PI3K regulatory subunit p85α, to sustain the ...
Changes in mice body weight during treatments are represented as mean±SEM (n = 6 mice per group). There is no significant difference between groups. d–f Representative images show that protein lysates derived from day 37 PDX tumor samples treated with vehicle, verteporfin, torin1 and ...
CITE-Seq and CROP-seq are new technologies that combine single-cell RNA sequencing with cell phenotype research and CRISPR screening, which are of great significance for tumor cell epitope protein index and tumor drug-resistant cell screening. For a long time, tumor heterogeneity has been an ...
Cells were plated in a six-well plate at a density of 0.3 × 106 cells per well and were allowed to attach overnight. After 24 h of treatment with PEITC, cells were harvested and lysed as described under western blotting analysis to collect and estimate the protein. The protein was transf...
Due to the small sample size in many subgroups, the predictive value of ALG3 based on different TP53 status and the tumor types needs to be investigated in a larger sample size in the future studies. It is interesting that breast cancer cell lines with TP53 mutation tended to overexpress ALG...
Nerve cells in the brain and throughout the body can turbocharge tumor growth — a finding that not only expands conventional ideas about the nervous system but points to novel therapeutic targets for a range of malignancies.大脑和全身的神经细胞可以加速肿瘤生长--这一发现不仅拓展了人们对神经系统的...
Thioredoxin-interacting protein (TXNIP) is commonly considered a master regulator of cellular oxidation, regulating the expression and function of Thioredoxin (Trx). Recent work has identified that TXNIP has a far wider range of additional roles: from re