产品活性:BAPTA-AM 是一种可透过细胞膜的钙螯合剂 (Ca2+ chelator)。在 HEK 293 细胞中,BAPTA-AM 阻断 hERG,hKv1.3 和 hKv1.5 通道,IC50 分别为 1.3,1.45 和 1.23 μM。 生物活性:BAPTA-AM 是一种众所周知的膜渗透性 Ca2+ 螯合剂。 BAPTA-AM 抑制 HEK 293 细胞中的 hERG 通道、hKv1.3 和hKv1.5...
Tymianski M, Wallace MC, Spigelman I, Uno M, Carlen PL, Tator CH, Charlton MP (1993) Cell-permeant Ca2+ chelators reduce early ex- citotoxic and ischemic neuronal injury in vitro and in vivo. Neuron 11:221-235.Cell-permeant Ca 2+ chelators reduce early excitotoxic and ischemic ...
Ca2+ chelator counteracted the effect of Ca2+ ionophore in ESCs and EM1 cells.Kazuya, KusamaMikihiro, YoshieKazuhiro, TamuraKazuhiko, ImakawaKeiichi, IsakaEiichi, Tachikawa
Similar results were obtained using another Ca2 þ chelator, BAPTA-AM (1,2-bis-(o-Aminophenoxy)-ethane-N,N,N',N'-tet- raacetic acid, tetraacetoxymethyl ester), which is cell-perme- able and acts as intracellular Ca2 þ sponge (Supplementary Figures S1A and B). Thus, inhibition of...
To further explore the underlying mechanism of decreased CaMKII activation inTmco1−/−osteoblasts, we treated WT andTmco1−/−osteoblasts with BAPTA-AM and EGTA-AM, two intracellular Ca2+chelators with different kinetics, to compare the changes in CaMKII phosphorylation in these cells. Under...
(E) Summary data to of the the total charge entered half-activation time tchormoupgahreLdTbCeCtwsedeunri5n0g µtMheEpGreTpAulasned(QCa 50 µM BAPTA as Ca2+ chelators (*P < 0.05). apamin-sensitive and progressive increase in K+ currents in both buffer conditions. However, the...
Pretreatment of the cells with the Ca2+ chelators BAPTA-AM or EGTA, as well as the Ca2+ influx inhibitor gadolinium, resulted in a partial decrease of the ERK response. Furthermore, the calmodulin antagonists W-7, trifluoperazine, and J-8 markedly decrea
To test this hypothesis, we used Ca2+ chelators: BAPTA suppresses rises in Ca2+ very near the channel pore, whereas EGTA has a 100-fold slower on rate and thus allows a larger plume of elevated Ca2+ in a nanodomain near the channel while suppressing rises in bulk Ca2+ microns away. We...
LPS-triggered ENO-1 exteriorization was suppressed by pretreatment of MDA-MB-231 cells with the Ca2+ chelator BAPTA or an inhibitor of endoplasmic reticulum Ca2+-ATPase pump, cyclopiazonic acid. In line with these observations, the stromal interaction molecule (STIM) 1 and the calcium release-...
P. Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse. J. Neurosci. 11, 1496–1507 (1991). A classical paper that uses exogenous Ca2+ chelators with different binding rates to probe the distance between Ca2+ source and Ca2+ sensor at the squid ...