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IT is now established that a change in intracellular free Ca2+forms a vital link in the action of certain hormones and neuro-transmitters as well as in excitation-secretion and excitation-contraction coupling1–5. Here we report an increase in intracellular free Ca2+(Cai2+), brought about by ...
4A). This result was also achieved by using Ca2+-free medium to fully remove Ca2+ (Fig. 5C and Supplementary Fig. 4B). Using immunofluorescence, we confirmed that both EDTA and Ca2+-free medium inhibited Nrf2 nuclear translocation (Fig. 5E). It is worth noting that the ratio of Nrf2-...
MCA截短蛋白是Ca2+可渗透通道 该研究首先利用添加了脂质体的小麦胚芽无细胞表达系统（liposome-supplemented wheat germ cell-free translation system）在体外合成和纯化了带6xHis标记的MCA1和MCA2截短蛋白（N端173个氨基酸），并证实了它们可以形成同源四聚体。那么，含合成MCA的脂质体是否可以介导Ca2+的渗透吸收呢？
HEK-APP-Orai1 cells transfected with STIM1D76A exhibited the most prominent elevations in [Ca2+]i levels to such a degree that resting cytosolic Ca2+ levels were significantly elevated even in nominally Ca2+-free buffer. This elevation of basal Ca2+ levels suggests significant alterations in ...
In skeletal muscle, the distance between CRUs depends on the length of free T tubule intercalated between the junctional T tubule segments forming triads (see Fig. 2, A–C). Very fast- and fast-twitch fibers have closely spaced CRUs (average minimum distance ∼100 nm, or three times the...
Differentiated STIM1-KO cells showed a significant decrease of mitochondrial respiratory chain complex I activity, mitochondrial inner membrane depolarization, reduced mitochondrial free Ca2+ concentration, and higher levels of senescence as compared with wild-type cells. In parallel, STIM1-KO cells ...
Free Ca2+ was also calculated from the stability constants of the chelates at pH 4.0 and pH 6.0. The strongest chelator at pH 4.8 was citrate, reducing free Ca2+ from 10 to 0.5 mol m3, while isocitrate and malate decreased ionized Ca2+ to 25% and 5O%, respectively. At pH 4.0 ...
If Ca2+ uptake is effectively driven by a pH-dependent mechanism, but the resting lysosomal Ca2+ permeability is low, bafilomycin A1 and/or concanamycin will require longer time to cause a robust drop in the intraluminal free Ca2+ concentration, as elegantly discussed in [14]. Notably, HEK293...
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