Persad S,Gupta KK,Dhalla NS.Status of Ca(2+)-channels in hearts perfused with Ca(2+)-free medium as well as upon reperfusion (Ca(2+)-paradox). Journal of Molecular and Cellular Cardiology . 1995Persad, S, Gupta, KK, Dhalla, NS (1995) Status of Ca2+-channels in hearts perfused ...
rich environments in the human body and in the culture - for example, 45–86 nM in the RBC cytosol, ~40 μM in the PV space, and ~1 mM in the human plasma25,26 - we fur- ther evaluated the effect of CPA and TG in Ca2+-free medium after selective membrane permeabilization...
Furthermore, αLA-mediated GLP-1 secretion by STC-1 cells was significantly inhibited by Ca2+- free medium, implying that Ca2+ influx drives FFA4-mediated GLP-1 secretion[13]. However, nifedipine (an L-type Ca2+ chan- nel blocker), thapsigargin, and U-73122 (a phospholipase C inhibitor...
4B). Using immunofluorescence, we confirmed that both EDTA and Ca2+-free medium inhibited Nrf2 nuclear translocation (Fig. 5E). It is worth noting that the ratio of Nrf2-positive cells in the nucleus in the EDTA-treated and Ca2+-free group were similar to the control group, suggesting that...
Rat neonatal ventricular myocytes were cultured in the standard serum-free medium (no insulin) for 48 h. This resulted in Ͼ90% of the cardiomyocytes being arrested in the G0-G1 phase. After this process, cells were incubated in the same medium as for electrophysiologic studies, supple- ...
(A) In Ca2+-free PSS the induced rise in the EC [Ca2+]i is reduced only with CP94. (B) When Ca2+ was included in the medium all the chelators reduced the [Ca2+]i and the (C) vasodilatation responses. (5 vessels from 5 animals; paired t test *p<0.05, **p<0.01). In the ...
Measurement Reading Mode Auto Read (Fast, Medium, Slow), Timed, Continuous Reading Prompts Reading, Stable, Locked Temp. Compensation ATC, MTC Data management Data Storage 500 results each GLP Features Yes Inputs Conductivity with Temp. Probe 5-pin aviation conn...
The effect of ET-1 persisted when Ca2+ was removed from superfusion medium (mean Δ[Ca2+]i in Ca2+-free medium, 233 ± 40 nmol/L above basal, N = 8, Figure 1d, right). Download: Download full-size image Figure 1. Dose-dependent [Ca2+]i increases induced by basolateral superfusion...
To explain that bronchial smooth muscle undergoes sustained agonist-induced contractions in a Ca(2+)-free medium, we hypothesized that caveolae in the plas... PJ Darby,CY Kwan,EE Daniel - 《American Journal of Physiology Lung Cellular & Molecular Physiology》 被引量: 407发表: 2000年 Store-ope...
The reaction was quenched with ice-cold FBS, and the cells were plated after washing in complete medium containing 10% FBS, 100 U/mL penicillin, 25 mM HEPES, and nonessential amino acids. T-cell stimulation was either performed in wells coated with 10 µg/mL anti-human CD3 (UC...