产品活性:BAPTA-AM 是一种可透过细胞膜的钙螯合剂 (Ca2+ chelator)。在 HEK 293 细胞中,BAPTA-AM 阻断 hERG,hKv1.3 和 hKv1.5 通道,IC50 分别为 1.3,1.45 和 1.23 μM。 生物活性:BAPTA-AM 是一种众所周知的膜渗透性 Ca2+ 螯合剂。 BAPTA-AM 抑制 HEK 293 细胞中的 hERG 通道、hKv1.3 和hKv1.5...
Calcium chelator BAPTA-AM protects against iron overload-induced chondrocyte mitochondrial dysfunction and cartilage degeneration. Int J Mol Med. 2021. https://doi.org/10.3892/ijmm.2021.5029. Article PubMed PubMed Central Google Scholar Kegelman CD, et al. YAP and TAZ promote periosteal osteoblast...
The Ca2+ chelator BAPTA-AM completely inhibited motility of MEFs stimulated with cl-CD95L (Figure 2d), confirming that Ca2+ was instrumental in this cellular process. ABT-199 is a BH3 mimetic that selectively targets Bcl-2.26 ABT-199 treatment abrogated the residual intracellular Ca2+ response...
Similar results were obtained using another Ca2 þ chelator, BAPTA-AM (1,2-bis-(o-Aminophenoxy)-ethane-N,N,N',N'-tet- raacetic acid, tetraacetoxymethyl ester), which is cell-perme- able and acts as intracellular Ca2 þ sponge (Supplementary Figures S1A and B). Thus, inhibition of...
Pretreatment of the cells with the Ca2+ chelators BAPTA-AM or EGTA, as well as the Ca2+ influx inhibitor gadolinium, resulted in a partial decrease of the ERK response. Furthermore, the calmodulin antagonists W-7, trifluoperazine, and J-8 markedly decrea
BAPTA/AM (an intracellular Ca2+ chelator), dantrolene and cyclosporine A did not reverse the effect of AMA on ROS release. We also investigated whether intracellular calcium release inhibitor and antioxidant protect against AMA-induced bone resorbing cytokine release. Trolox prevented the release of ...
The intracellular Ca2 þ -chelator BAPTA-AM (5 mM) was pre-incubated with IB3-1 cells for 30 min. (d) The histograms show the changes in the basal [Ca2 þ ] in IB3-1 cells after 48 h of VX- 809 (5 mM) treatment; n ¼ 35 of independent experiments. (e,f) Levels of ...
Preincubation of the cells with the Ca2+ chelator BAPTA-AM prevented the stimulation of NHE3 by ANG II (Fig. 5, B and C), suggesting the obligatory role of Ca2+. Similarly, 2-APB completely blocked the effect of ANG II (Fig. 5, D and E). Interestingly, in the experiment using ...
(and therefore spontaneous activity) would be to apply a Ca2+ chelator, such as BAPTA, to the cytosol to lower [Ca2+]. As illustrated in Fig. 5, application of membrane-permeant BAPTA AM (10 μM), whether by rapid switch (n= 6; data not shown) or by superfusion (n= 6), caused...
Pre-incubation with the cell-permeable Ca2 þ -chelator BAPTA-AM was found to dose-dependently inhibit inflammasome activation by ex[Ca2 þ ] (Fig. 2m), thereby corroborating a crucial role of intracellular calcium accumulation, which has also been shown to be required for other inflammasome ...