产品活性:BAPTA-AM 是一种可透过细胞膜的钙螯合剂 (Ca2+ chelator)。在 HEK 293 细胞中,BAPTA-AM 阻断 hERG,hKv1.3 和 hKv1.5 通道,IC50 分别为 1.3,1.45 和 1.23 μM。 生物活性:BAPTA-AM 是一种众所周知的膜渗透性 Ca2+ 螯合剂。 BAPTA-AM 抑制 HEK 293 细胞中的 hERG 通道、hKv1.3 和hKv1.5...
Moreover, the intracellular Ca2+ chelator BAPTA-AM decreased the phosphorylation of p38 MAPK and CREB but failed to reduce PKA activation. In summary, p38 MAPK/CREB activation via PKA activation and increased cellular Ca2+ helped to alleviate Mn-induced neuronal apoptosis via BDNF regulation. These...
Calcium chelator BAPTA-AM protects against iron overload-induced chondrocyte mitochondrial dysfunction and cartilage degeneration. Int J Mol Med. 2021. https://doi.org/10.3892/ijmm.2021.5029. Article PubMed PubMed Central Google Scholar Kegelman CD, et al. YAP and TAZ promote periosteal osteoblast...
(c) Changes in IL-1b and IL-18 release in BAPTA-AM-pretreated IB3-1 cells after PAO-1 infection. The intracellular Ca2 þ -chelator BAPTA-AM (5 mM) was pre-incubated with IB3-1 cells for 30 min. (d) The histograms show the changes in the basal [Ca2 þ ] in IB3-1 cells...
Similar results were obtained using another Ca2 þ chelator, BAPTA-AM (1,2-bis-(o-Aminophenoxy)-ethane-N,N,N',N'-tet- raacetic acid, tetraacetoxymethyl ester), which is cell-perme- able and acts as intracellular Ca2 þ sponge (Supplementary Figures S1A and B). Thus, inhibition of...
To further explore the involvement of Ca2+ in LPS-triggered transport of ENO-1 to the cell surface, the levels of intracellular Ca2+ were depleted by pretreatment of MDA-MB-231 cells with the Ca2+ chelator BAPTA. As depicted in Fig. 6C, BAPTA blocked LPS-driven ENO-1 translocation. ...
(a selective OX1R antagonist), TCSOX229 (a selective OX2R antagonist), Bis-IV (a PKC inhibitor), PMA (a PKC agonist), Rp-cAMP (a PKA inhibitor), or BAPTA (Ca2 + chelator). Results The isolated neurons maintained good morphologies with diverse shapes of cell body and long protrusions...
By linking two fura fluorophores with one BAPTA chelator, we have produced bis-fura-2, a Ca2+ indicator that exhibits approximately twice the absorptivity of fura-2. Bis-fura-2 has a Kd for Ca2+ of ~370 nM and ~525 nM in the absence and ...
[Ca2+] to a level below the minimum recorded between spontaneous Ca2+ transients. The Ca2+ chelators BAPTA and EGTA, which would be expected to greatly reduce the minimum level of cytosolic Ca2+ (regardless of whether the major source was from the SR or Ca2+ entry across the sarcolemma) ...
The Ca2+ chelator BAPTA-AM completely inhibited motility of MEFs stimulated with cl-CD95L (Figure 2d), confirming that Ca2+ was instrumental in this cellular process. ABT-199 is a BH3 mimetic that selectively targets Bcl-2.26 ABT-199 treatment abrogated the residual intracellular Ca2+ response...