产品活性:BAPTA-AM 是一种可透过细胞膜的钙螯合剂 (Ca2+ chelator)。在 HEK 293 细胞中,BAPTA-AM 阻断 hERG,hKv1.3 和 hKv1.5 通道,IC50 分别为 1.3,1.45 和 1.23 μM。 生物活性:BAPTA-AM 是一种众所周知的膜渗透性 Ca2+ 螯合剂。 BAPTA-AM 抑制 HEK 293 细胞中的 hERG 通道、hKv1.3 和hKv1.5...
Intracellular Ca2+ chelators prevent DNA damage and protect hepatoma 1C1C7 cells from quinone-induced cell killing. Free Radic Res Commun. 1990; 8:347-54.DYPBUKT J.M., THOR H. & NICOTERA P. (1900) Intracellular Ca2 + chelators prevent DNA damage and protect hepatoma 1 C 17 cells from...
Ca2+ chelator (BAPTA) reduced neuronal NA release by 65%, confirming the effectiveness of the loading protocol. Release of NA independent of nerve activity occurred in hearts subjected to a 40 min period of anoxic, substrate-free and nominal Ca2+-free perfusion. Loading hearts with BAPTA prior...
A method based on the osmotic lysis of pinocytic vesicles—a technique that can be conveniently implemented using our Influx pinocytic cell-loading reagent (I14402, Chelators, Calibration Buffers, Ionophores and Cell-Loading Reagents—Section 1...
Ca2+ chelator counteracted the effect of Ca2+ ionophore in ESCs and EM1 cells.Kazuya, KusamaMikihiro, YoshieKazuhiro, TamuraKazuhiko, ImakawaKeiichi, IsakaEiichi, Tachikawa
Similar results were obtained using another Ca2 þ chelator, BAPTA-AM (1,2-bis-(o-Aminophenoxy)-ethane-N,N,N',N'-tet- raacetic acid, tetraacetoxymethyl ester), which is cell-perme- able and acts as intracellular Ca2 þ sponge (Supplementary Figures S1A and B). Thus, inhibition of...
Calcein (C481, Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7) is a relatively low-affinity Ca2+ chelator. The dissociation constants for both the Ca2+ and Mg2+ complexes of calcein at physiological pH are about 10-...
To further explore the underlying mechanism of decreased CaMKII activation inTmco1−/−osteoblasts, we treated WT andTmco1−/−osteoblasts with BAPTA-AM and EGTA-AM, two intracellular Ca2+chelators with different kinetics, to compare the changes in CaMKII phosphorylation in these cells. Under...
We first tested the role of Ca2+signaling in macrophage efferocytosis. BMDMs were loaded with either BAPTA-AM or EGTA-AM Ca2+chelators prior to incubation with labeled apoptotic cells. Although BAPTA and EGTA have similar affinities for Ca2+, they exhibit very different kinetics of Ca2+-binding...
Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA Nagai, Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA, Biochimie 81 (1999) 909-913; ... M Wachi,N Iwai,A Kunihisa...