有研究表明EA可诱导人脐静脉内皮细胞的动脉粥样硬化;通过促进炎症和氧化应激(OS)加速小鼠动脉粥样硬化;可诱导多种细胞凋亡,增加糖尿病小鼠的内质网应激(ERS)和OS,并激活巨噬细胞中的NLRP3炎性小体,但其在慢性疾病中的具体致病机制有待...
有研究表明EA可诱导人脐静脉内皮细胞的动脉粥样硬化;通过促进炎症和氧化应激(OS)加速小鼠动脉粥样硬化;可诱导多种细胞凋亡,增加糖尿病小鼠的内质网应激(ERS)和OS,并激活巨噬细胞中的NLRP3炎性小体,但其在慢性疾病中的具体致病机制有...
Intracellular Ca2+ increase by ω-3 PUFAs in human colonic epithelial cells Because FFA4 couples to G proteins and increases [Ca2+]i in FFA4-overexpressing cells, this Ca2+ response was measured in colonic epithelial cells endogenously expressing FFA4. As shown Figure 2A, the ω-3 PUFAs, ...
内质网应激(ER stress)是由于内源或外源的刺激(如Ca2+稳态的破坏、病毒感染和氧化还原平衡等)导致内质网中错误折叠蛋白的积累而引发。为了应对细胞遭受的内质网应激,细胞会产生未折叠蛋白反应(Unfolded protein response, UPR)[1]。细胞通过启动UPR来抑制翻译过程,减弱蛋白质合成从而减轻内质网蛋白合成负担、同时促进内质网...
[5] J. Shan, A. Kushnir, M.J. Betzenhauser, S. Reiken, J. Li, S.E. Lehnart, N. Lindegger, M. Mongillo, P.J. Mohler, A.R. Marks, Phosphorylation of the ryanodine receptor mediates the cardiac fight or flight response in mice, J Clin Invest, 120 (2010) 4388-4398. ...
(baseline) after nearly 220 s. Finally, the addition of ionomycin elicited the maximal Ca2+response. In 0 mM extracellular Ca2+, the release of intracellular stored Ca2+from the ER was comparable in amplitude to the SOCE seen in 2 mM Ca2+, but unsurprisingly, [Ca2+]idecayed to ...
However, we found that in the Ca2+ response to caffeine, the function of this Ca2+ release machinery, most probably RyRs, might be immature, because the mean amplitude of the Ca2+ transients induced by caffeine was much lower than that induced by ATP (Figure 3A). Sub-cloning these ...
(measured with indo 1) was 0.55 ± 0.03 (Δ ratio,n = 26) in myocytes pretreated with 1.7 mm[Ca2+]o and 0.79 ± 0.07 (n= 26) in myocytes pretreated with 10 mm[Ca2+]o, indicating an increase in Ca2+ response amplitude similar to that obtained in Ca2+-containing solutions (about ...
The mitochondrial Ca2 + peak in response to ER Ca2 + mobilization (1st peak) was significantly higher in cells overexpressing parkin (peak values: 38.31 ± 2.39 μM, n = 15) compared to vector transfected control cells (peak value: 27.14 ± 1.29 μM, n = 25; p < 0.0001). The Ca2 ...
(3D representative of calcium transients in 300 s over defined threshold level, Line 4CX1), and their significant change showing the spontaneous Ca2+ transient activity of the cells and their response after the application of DL-AP5 (50 μM, a) and CNQX (50 μM, b), Nifedipine (...